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Resumen
The molecule Dimethyl sulfoxide is widely used as drug solvent. However, its antioxidant property was poorly explored. In this study, we evaluated the effect of DMSO supplementation during oocyte in vitro maturation (IVM) on embryo development and quality. Bovine oocytes were matured with different DMSO concentrations (0, 0.1, 0.25, 0.5, 0.75, 1 and 10% v:v) followed by in vitro fertilization. Subsequently, quality indicators such as gene expression of [ver mas...]
dc.contributor.authorYnsaurralde Rivolta, Amanda Eugenia
dc.contributor.authorSuvá, Mariana
dc.contributor.authorLuchetti, Carolina Griselda
dc.contributor.authorBevacqua, Romina Jimena
dc.contributor.authorMunilla, Sebastian
dc.contributor.authorRodriguez-Alvarez, Lleretny
dc.contributor.authorVelasquez, Alejandra
dc.contributor.authorBriski, Olinda
dc.contributor.authorLombardo, Daniel
dc.contributor.authorSalamone, Daniel Felipe
dc.date.accessioned2020-06-09T13:21:44Z
dc.date.available2020-06-09T13:21:44Z
dc.date.issued2020-05
dc.identifier.issn0093-691X
dc.identifier.issn1879-3231
dc.identifier.otherhttps://doi.org/10.1016/j.theriogenology.2020.02.045
dc.identifier.urihttp://hdl.handle.net/20.500.12123/7380
dc.identifier.urihttps://www.sciencedirect.com/science/article/abs/pii/S0093691X20301576
dc.description.abstractThe molecule Dimethyl sulfoxide is widely used as drug solvent. However, its antioxidant property was poorly explored. In this study, we evaluated the effect of DMSO supplementation during oocyte in vitro maturation (IVM) on embryo development and quality. Bovine oocytes were matured with different DMSO concentrations (0, 0.1, 0.25, 0.5, 0.75, 1 and 10% v:v) followed by in vitro fertilization. Subsequently, quality indicators such as gene expression of SOX2, OCT4, CDX2, SOD1, oocyte and embryo redox status and DNA damage were evaluated. Polar body extrusion and blastocyst rates increased with 0.5% v:v DMSO. Moreover, first polar body extrusion and blastocyst rates did not increase with 1%, and 10% of DMSO reduced polar body extrusion and did not produce blastocyst. Optimal concentration of DMSO for the use on the maturation was estimated at around 0.45% v:v. Supplementation with 0.5% v:v DMSO has not affected mRNA abundance of genes key in blastocyst, however 0.75% increased gene expression of OCT4 and SOX2. Oocytes matured with 0.5% v:v DMSO and blastocyst from DMSO group showed reduced lipid peroxidation respect control. Total Glutathione concentrations increased in blastocyst stage in DMSO group. DMSO increased the total cell number of blastocysts but not TUNEL positive cells. In conclusion, our results suggest that low DMSO concentrations used during bovine oocytes in vitro maturation increases the maturation, as well as the blastocyst rate and its quality, without demonstrating deleterious effect on embryo cells.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherElsevieres_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceTheriogenology 148 : 140-148 (May 2020)es_AR
dc.subjectGanado Bovinoes_AR
dc.subjectCattleeng
dc.subjectSuplementoses_AR
dc.subjectSupplementseng
dc.subjectEstrés Oxidativoes_AR
dc.subjectOxidative Stresseng
dc.subjectÓvuloes_AR
dc.subjectOvaeng
dc.subjectExperimentación in Vitroes_AR
dc.subjectIn Vitro Experimentationeng
dc.subject.otherOocyteseng
dc.titleDMSO supplementation during in vitro maturation of bovine oocytes improves blastocyst rate and qualityes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEEA Mercedeses_AR
dc.description.filFil: Ynsaurralde Rivolta, Amada Eugenia. Universidad de Buenos Aires. Facultad de Agronomía; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Suvá, Mariana. Universidad de Buenos Aires. Facultad de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Luchetti, Carolina Griselda. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal (INITRA). Cátedra de Histología y Embriología, Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Bevacqua, Romina Jimena. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Munilla, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal; Argentinaes_AR
dc.description.filFil: Rodriguez-Alvarez, Lleretny. Universidad de Concepción. Facultad de Ciencias Veterinarias; Chilees_AR
dc.description.filFil: Velasquez, Alejandra. Universidad de Concepción. Facultad de Ciencias Veterinarias; Chilees_AR
dc.description.filFil: Briski, Olinda. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Lombardo, Daniel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal (INITRA). Cátedra de Histología y Embriología, Argentina.es_AR
dc.description.filFil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.subtypecientifico


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