Show simple item record

resumen

Abstract
Detection of bovine viral diarrhea virus (BVDV) in aborted fetus samples is often difficult due to tissue autolysis and inappropriate sampling. Studies assessing different methods for BVDV identification in fetal specimens are scarce. The present study evaluated the agreement between different diagnostic techniques to detect BVDV infections in specimens from a large number of bovine aborted fetuses and neonatal deaths over a period of 22 years. [ver mas...]
dc.contributor.authorSpetter, Lucas Maximiliano
dc.contributor.authorLouge Uriarte, Enrique Leopoldo
dc.contributor.authorArmendano, Joaquín Ignacio
dc.contributor.authorMorrell, Eleonora Lidia
dc.contributor.authorCanton, German Jose
dc.contributor.authorVerna, Andrea Elizabeth
dc.contributor.authorDorsch, Matías
dc.contributor.authorPereyra, Susana Beatriz
dc.contributor.authorOdeon, Anselmo Carlos
dc.contributor.authorSaliki, Jeremiah
dc.contributor.authorGonzález Altamiranda, Erika
dc.date.accessioned2020-06-03T17:41:33Z
dc.date.available2020-06-03T17:41:33Z
dc.date.issued2020-05-16
dc.identifier.issn1517-8382
dc.identifier.otherhttps://doi.org/10.1007/s42770-020-00296-z
dc.identifier.urihttp://hdl.handle.net/20.500.12123/7354
dc.identifier.urihttps://link.springer.com/article/10.1007/s42770-020-00296-z
dc.description.abstractDetection of bovine viral diarrhea virus (BVDV) in aborted fetus samples is often difficult due to tissue autolysis and inappropriate sampling. Studies assessing different methods for BVDV identification in fetal specimens are scarce. The present study evaluated the agreement between different diagnostic techniques to detect BVDV infections in specimens from a large number of bovine aborted fetuses and neonatal deaths over a period of 22 years. Additionally, genetic, serological, and pathological analyses were conducted in order to characterize BVDV strains of fetal origin. Samples from 95 selected cases from 1997 to 2018 were analyzed by antigen-capture ELISA (AgELISA), nested RT-PCR (RT-nPCR), and real-time RT-PCR (RT-qPCR). In addition, amplification and sequencing of the 5'UTR region were performed for phylogenetic purposes. Virus neutralization tests against the BVDV-1a, BVDV-1b, and BVDV-2b subtypes were conducted on 60 fetal fluids of the selected cases. Furthermore, the frequency and severity of histopathological lesions were evaluated in BVDV-positive cases. This study demonstrated that RT-nPCR and RT-qPCR were more suitable than AgELISA for BVDV detection in fetal specimens. However, the agreement between the two RT-PCR methods was moderate. The BVDV-1b subtype was more frequently detected than the BVDV-1a and BVDV-2b subtypes. Neutralizing antibodies to any of the three subtypes evaluated were present in 94% of the fetal fluids. Microscopically, half of the BVDV-positive cases showed a mild non-suppurative inflammatory response. These results emphasize the need to consider different methods for a diagnostic approach of BVDV associated to reproductive losses.eng
dc.formatapplication/pdfeng
dc.language.isoenges_AR
dc.publisherSpringeres_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.sourceBrazilian journal of microbiology (2020)es_AR
dc.subjectAbortoes_AR
dc.subjectAbortioneng
dc.subjectDiagnósticoes_AR
dc.subjectDiagnosiseng
dc.subjectFilogeniaes_AR
dc.subjectPhylogenyeng
dc.subjectPatologíaes_AR
dc.subjectPathologyeng
dc.subject.otherBVDV
dc.titleDetection methods and characterization of bovine viral diarrhea virus in aborted fetuses and neonatal calves over a 22-year periodes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenEEA Balcarcees_AR
dc.description.filFil: Spetter, Maximiliano Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentinaes_AR
dc.description.filFil: Louge Uriarte, Enrique Leopoldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentinaes_AR
dc.description.filFil: Armendano, Joaquín Ignacio. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentinaes_AR
dc.description.filFil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentinaes_AR
dc.description.filFil: Cantón, Germán José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentinaes_AR
dc.description.filFil: Verna, Andrea Elizabeth . Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.es_AR
dc.description.filFil: Dorsch, Matías A. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.es_AR
dc.description.filFil: Pereyra, Susana Beatriz . Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.es_AR
dc.description.filFil: Odeón, Anselmo C. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.es_AR
dc.description.filFil: Saliki, Jeremiah T. University of Georgia. Athens Veterinary Diagnostic Laboratory; Estados Unidoses_AR
dc.description.filFil: González Altamiranda, Erika A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentinaes_AR
dc.subtypecientifico


Files in this item

Thumbnail

This item appears in the following Collection(s)

common

Show simple item record