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Resumen
Polyphenol Oxidases (PPOs) catalyze the conversion of phenolic substrates to quinones, leading to the formation of dark-colored precipitates in fruits and vegetables. This process, known as enzymatic browning, is the cause of undesirable changes in organoleptic properties and the loss of nutritional quality in plant-derived products. In potato (Solanum tubersoum L.), PPOs are encoded by a multi-gene family with different expression patterns. Here, we have [ver mas...]
dc.contributor.authorGonzalez, Matías Nicolás
dc.contributor.authorMassa, Gabriela Alejandra
dc.contributor.authorAndersson, Mariette
dc.contributor.authorTuresson, Helle
dc.contributor.authorOlsson, Niklas
dc.contributor.authorFält, Ann-Sofie
dc.contributor.authorStorani, Leonardo
dc.contributor.authorDecima Oneto, Cecilia Andrea
dc.contributor.authorHofvander, Per
dc.contributor.authorFeingold, Sergio Enrique
dc.date.accessioned2020-03-03T16:45:34Z
dc.date.available2020-03-03T16:45:34Z
dc.date.issued2020-01-09
dc.identifier.issn1664-462X
dc.identifier.otherhttps://doi.org/10.3389/fpls.2019.01649
dc.identifier.urihttp://hdl.handle.net/20.500.12123/6872
dc.identifier.urihttps://www.frontiersin.org/articles/10.3389/fpls.2019.01649/full
dc.description.abstractPolyphenol Oxidases (PPOs) catalyze the conversion of phenolic substrates to quinones, leading to the formation of dark-colored precipitates in fruits and vegetables. This process, known as enzymatic browning, is the cause of undesirable changes in organoleptic properties and the loss of nutritional quality in plant-derived products. In potato (Solanum tubersoum L.), PPOs are encoded by a multi-gene family with different expression patterns. Here, we have studied the application of the CRISPR/Cas9 system to induce mutations in the StPPO2 gene in the tetraploid cultivar Desiree. We hypothesized that the specific editing of this target gene would result in a lower PPO activity in the tuber with the consequent reduction of the enzymatic browning. Ribonucleoprotein complexes (RNPs), formed by two sgRNAs and Cas9 nuclease, were transfected to potato protoplasts. Up to 68% of regenerated plants contained mutations in at least one allele of the target gene, while 24% of edited lines carried mutations in all four alleles. No off-target mutations were identified in other analyzed StPPO genes. Mutations induced in the four alleles of StPPO2 gene, led to lines with a reduction of up to 69% in tuber PPO activity and a reduction of 73% in enzymatic browning, compared to the control. Our results demonstrate that the CRISPR/Cas9 system can be applied to develop potato varieties with reduced enzymatic browning in tubers, by the specific editing of a single member of the StPPO gene family.eng
dc.formatapplication/pdfeng
dc.language.isoenges_AR
dc.publisherFrontiers Media, Switzerlandeng
dc.rightsinfo:eu-repo/semantics/openAccesseng
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.sourceFrontiers in plant science 10 : article 1649. (09 January 2020)es_AR
dc.subjectPapaes_AR
dc.subjectPotatoeseng
dc.subjectSolanum Tuberosumes_AR
dc.subjectPardeamiento Enzimáticoes_AR
dc.subjectEnzymic Browningeng
dc.subjectCatecol Oxdasaes_AR
dc.subjectCatechol Oxidaseeng
dc.subjectGenomases_AR
dc.subjectGenomeseng
dc.subject.otherPolifenol Oxidasees_AR
dc.titleReduced enzymatic browning in potato tubers by specific editing of a Polyphenol oxidase gene via Ribonucleoprotein complexes delivery of the CRISPR/Cas9 System.es_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.rights.licenseCreative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.description.origenEEA Balcarcees_AR
dc.description.filFil: González, Matías Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentinaes_AR
dc.description.filFil: Massa, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agraria; Argentina.es_AR
dc.description.filFil: Andersson, Mariette. Swedish University of Agricultural Sciences, Department of Plant Breeding; Sueciaes_AR
dc.description.filFil: Turesson, Helle. Swedish University of Agricultural Sciences, Department of Plant Breeding; Sueciaes_AR
dc.description.filFil: Olsson, Niklas. Swedish University of Agricultural Sciences, Department of Plant Breeding; Sueciaes_AR
dc.description.filFil: Fält, Ann-Sofie. Swedish University of Agricultural Sciences, Department of Plant Breeding; Sueciaes_AR
dc.description.filFil: Storani, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentinaes_AR
dc.description.filFil: Decima Oneto, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentinaes_AR
dc.description.filFil: Hofvander, Per. Swedish University of Agricultural Sciences, Department of Plant Breeding; Sueciaes_AR
dc.description.filFil: Feingold, Sergio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.es_AR
dc.subtypecientifico


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