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Abstract
Xanthomonas fuscans ssp. aurantifolii group C strains exhibit host specificity on different citrus species. The strains possess a type III effector, AvrGf2, belonging to the XopAG effector gene family, which restricts host range on citrus. We dissected the modular nature and mode of action of AvrGf2 in grapefruit resistance. XopAG effectors possess characteristic features, such as a chloroplast localization signal, a cyclophilin-binding domain [ver mas...]
dc.contributor.authorGochez, Alberto Martin
dc.contributor.authorShantharaj, Deepak
dc.contributor.authorPotnis, Neha
dc.contributor.authorZhou, Xiaofeng
dc.contributor.authorMinsavage, Gerald V.
dc.contributor.authorWhite, Frank F.
dc.contributor.authorWang, Nian
dc.contributor.authorHurlbert, Jason C.
dc.contributor.authorJones, Jeffrey B.
dc.date.accessioned2017-07-11T15:52:17Z
dc.date.available2017-07-11T15:52:17Z
dc.date.issued2017-04
dc.identifier.issn1364-3703
dc.identifier.otherDOI: 10.1111/mpp.12408
dc.identifier.urihttp://hdl.handle.net/20.500.12123/642
dc.identifier.urihttp://onlinelibrary.wiley.com/doi/10.1111/mpp.12408/epdf?r3_referer=wol&tracking_action=preview_click&show_checkout=1&purchase_referrer=www.ncbi.nlm.nih.gov&purchase_site_license=LICENSE_DENIED
dc.description.abstractXanthomonas fuscans ssp. aurantifolii group C strains exhibit host specificity on different citrus species. The strains possess a type III effector, AvrGf2, belonging to the XopAG effector gene family, which restricts host range on citrus. We dissected the modular nature and mode of action of AvrGf2 in grapefruit resistance. XopAG effectors possess characteristic features, such as a chloroplast localization signal, a cyclophilin-binding domain characteristic amino acid sequence motif (GPLL) and a C-terminal domain-containing CLNAxYD. Mutation of GPLL to AASL in AvrGf2 abolished the elicitation of the hypersensitive response (HR), whereas mutation of only the first amino acid to SPLL delayed the HR in grapefruit. Yeast two-hybrid experiments showed strong interaction of AvrGf2 with grapefruit cyclophilin (GfCyp), whereas AvrGf2-SPLL and AvrGf2-AASL mutants showed weak and no interaction, respectively. Molecular modeling and in silico docking studies for the cyclophilin–AvrGf2 interaction predicted the binding of citrus cyclophilins (CsCyp, GfCyp) to hexameric peptides spanning the cyclophilin-binding domain of AvrGf2 and AvrGf2 mutants (VAGPLL, VASPLL and VAAASL) with affinities equivalent to or better than a positive control peptide (YSPSA) previously demonstrated to bind CsCyp. In addition, the C-terminal domain of XopAG family effectors contains a highly conserved motif, CLNAxYD, which was identified to be crucial for the induction of HR based on site-directed mutagenesis (CLNAxYD to CASAxYD). Our results suggest a model in which grapefruit cyclophilin promotes a conformational change in AvrGf2, thereby triggering the resistance response.eng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.sourceMolecular plant pathology 18 (3) : 405-419. (2017)eng
dc.subjectEnfermedades de las Plantas
dc.subjectXanthomonas
dc.subjectGrapefruitseng
dc.subjectToronja
dc.subjectPomelo
dc.subjectGenes
dc.subjectGenética Molecular
dc.subjectMolecular Geneticseng
dc.subjectPlant Diseaseseng
dc.titleMolecular characterization of XopAG effector AvrGf2 from Xanthomonas fuscans ssp. aurantifolii in grapefruiteng
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenEEA Bella Vista
dc.gic154093
dc.description.filFil: Gochez, Alberto Martín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bella Vista; Argentina. University of Florida. Department of Plant Pathology; Estados Unidos
dc.description.filFil: Shantharaj, Deepak. University of Florida. Department of Plant Pathology; Estados Unidos
dc.description.filFil: Potnis, Neha. University of Florida. Department of Plant Pathology; Estados Unidos
dc.description.filFil: Zhou, Xiaofeng. University of Florida. Department of Microbiology and Cell Science; Estados Unidos
dc.description.filFil: Minsavage, Gerald V. University of Florida. Department of Plant Pathology; Estados Unidos
dc.description.filFil: White, Frank F. University of Florida. Department of Plant Pathology; Estados Unidos
dc.description.filFil: Wang, Nian. University of Florida. Department of Microbiology and Cell Science; Estados Unidos
dc.description.filFil: Hurlbert, Jason C. Winthrop University. Department of Chemistry, Physics and Geology, Citrus Research and Education Center; Estados Unidos
dc.description.filFil: Jones, Jeffrey B. University of Florida. Department of Plant Pathology; Estados Unidos
dc.subtypecientifico


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