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Resumen
Rabies is an important zoonosis with impact on livestock production. The diagnosis is usually based on the rabies virus detection in fresh or refrigerated brain samples by direct immunofluorescence (IF) performed on fixed brain smears. The aim of this study was to evaluate the performance of 6 monoclonal and 1 rabbit-polyclonal new antibodies for rabies diagnostic by using immunohistochemistry (IHC), which detects the agent on formalin fixed paraffin [ver mas...]
dc.contributor.authorJauregui, Gloria R.
dc.contributor.authorFontana, Diego
dc.contributor.authorMicheloud, Juan Francisco
dc.contributor.authorPrieto, Claudio
dc.contributor.authorDelgado, Fernando Oscar
dc.date.accessioned2019-11-07T17:18:37Z
dc.date.available2019-11-07T17:18:37Z
dc.date.issued2019-07
dc.identifier.issn1983-0246
dc.identifier.urihttps://bjvp.org.br/wp-content/uploads/2019/07/v12-n2-1.pdf
dc.identifier.urihttp://hdl.handle.net/20.500.12123/6311
dc.description.abstractRabies is an important zoonosis with impact on livestock production. The diagnosis is usually based on the rabies virus detection in fresh or refrigerated brain samples by direct immunofluorescence (IF) performed on fixed brain smears. The aim of this study was to evaluate the performance of 6 monoclonal and 1 rabbit-polyclonal new antibodies for rabies diagnostic by using immunohistochemistry (IHC), which detects the agent on formalin fixed paraffin embedded tissues samples. Tested with 2 positive and 2 negative cows for rabies at dilutions 1/200 and 1/1000, obtained immunostaining was strong for one monoclonal and weak for the polyclonal at both dilutions. For 3 monoclonals, the immunostaining was weaker at 1/1000 and was negative at both dilutions for 2 monoclonals. Unwanted background was absent and negative samples remained clear for all antibodies. When the first monoclonal was applied on sections of brain of 19 cows with Rabies and 41 control cows at 1/1000 dilution, immunohistochemistry recognized all positive samples and was negative for all control cows. The number of cases analyzed did not allow estimating sensitivity and specificity of the tested assay, but the correlation observed between IF and IHC in both positive and negative samples suggested that accuracy of the test might be good. The results indicated that the tested antibody can detect the rabies virus on formalin fixed tissue samples, and that immunohistochemistry can complement other confirmatory tests when those cannot be performed.eng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.publisherBrazilian Association of Veterinary Pathologyes_AR
dc.relationinfo:eu-repograntAgreement/INTA/PNSA/1115052/AR./Epidemiología y desarrollo de estrategias para la prevención y control de enfermedades que afectan la salud pública, enfermedades exóticas y limitantes del comercio internacional.es_AR
dc.rightsinfo:eu-repo/semantics/openAccesseng
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.sourceBrazilian journal of veterinary pathology 12 (2) : 27–32.(Julio 2019)es_AR
dc.subjectRabies Viruseng
dc.subjectVirus de la Rabiaes_AR
dc.subjectImmunohistochemistryeng
dc.subjectInmunohistoquímicaes_AR
dc.subjectDiagnostic Techniqueseng
dc.subjectTécnicas de Diagnosises_AR
dc.subjectCattleeng
dc.subjectGanado Bovinoes_AR
dc.titleEvaluation of new antibodies for the detection of rabies virus in formalin fixed brain tissue sampleseng
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.rights.licenseCreative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.description.origenInstituto de Patobiologíaes_AR
dc.description.filFil: Jauregui, Gloria R. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Fontana, Diego. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Biotecnológicoes_AR
dc.description.filFil: Micheloud, Juan Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta. Grupo de Trabajo de Patología, Epidemiología e Investigación Diagnóstica. Área de Sanidad Animal; Argentinaes_AR
dc.description.filFil: Prieto, Claudio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Biotecnológicoes_AR
dc.description.filFil: Delgado, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Universidad del Salvador. Carrera de Veterinaria; Argentinaes_AR
dc.subtypecientifico


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