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Abstract
Bovine campylobacteriosis (BC) is a venereal disease caused by Campylobacter fetus characterized by temporary infertility with mild endometritis, early embryonic death and occasional abortions. The objectives of this study were to describe and identify C. fetus in spontaneous bovine abortion on the basis of histopathological, immunohistochemical, lectinhistochemical and polymerase chain reaction (PCR) techniques. The most frequent foetal lesion was [ver mas...]
dc.contributor.authorMorrell, Eleonora Lidia
dc.contributor.authorBarbeito, Claudio Gustavo
dc.contributor.authorOdeon, Anselmo Carlos
dc.contributor.authorGimeno, Eduardo Juan
dc.contributor.authorCampero, Carlos Manuel
dc.date.accessioned2019-10-01T14:33:38Z
dc.date.available2019-10-01T14:33:38Z
dc.date.issued2011-04
dc.identifier.issn0936-6768
dc.identifier.issn1439-0531
dc.identifier.otherhttps://doi.org/10.1111/j.1439-0531.2010.01668.x
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/abs/10.1111/j.1439-0531.2010.01668.x
dc.identifier.urihttp://hdl.handle.net/20.500.12123/6030
dc.description.abstractBovine campylobacteriosis (BC) is a venereal disease caused by Campylobacter fetus characterized by temporary infertility with mild endometritis, early embryonic death and occasional abortions. The objectives of this study were to describe and identify C. fetus in spontaneous bovine abortion on the basis of histopathological, immunohistochemical, lectinhistochemical and polymerase chain reaction (PCR) techniques. The most frequent foetal lesion was neutrophilic bronchopneumonia and interstitial pneumonia. Other commonly observed lesions included non‐suppurative interstitial enteritis, hepatitis, pericarditis, myositis, myocarditis, and meningitis. In this study, C. fetus fetus was phenotypically classified in all bovine foetuses from lungs and abomasal fluids. Immunohistochemistry (IHC) staining revealed positive stained Campylobacter organisms with typical morphology. Lectin binding patterns not showed great differences between the infected and the non‐infected groups. The most important changes were a minor peanut agglutinin (PNA) and DBA binding in the alveolar cells of the lungs and DBA globet cells in some of the C. fetus–positive foetuses. Individual variations in each lectin binding pattern complicate the evaluation of the lectins results. All foetuses positive to IHC were positive by PCR. Better efficiency of PCR was obtained from abomasal fluids than from lung tissues. The association of culture and phenotypic techniques with histopathology, IHC and PCR allowed a better characterization and description of BC.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherWileyes_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceReproduction in Domestic Animals 46 (2) : 309-315 (April 2011)es_AR
dc.subjectGanado Bovinoes_AR
dc.subjectCattleeng
dc.subjectEnfermedades de los Animaleses_AR
dc.subjectAnimal Diseaseseng
dc.subjectCampylobacter fetuses_AR
dc.subjectAbortoes_AR
dc.subjectAbortioneng
dc.subjectHistopatologíaes_AR
dc.subjectHistopathologyeng
dc.subjectInmunohistoquímicaes_AR
dc.subjectImmunohistochemistryeng
dc.subjectPCRes_AR
dc.subjectDiagnósticoes_AR
dc.subjectDiagnosiseng
dc.subject.otherReacción en Cadena de la Polimerasaes_AR
dc.subject.otherPolymerase Chain Reactioneng
dc.titleHistopathological, Immunohistochemical, Lectinhistochemical and Molecular Findings in Spontaneous Bovine Abortions by Campylobacter fetuses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEEA Balcarcees_AR
dc.description.filFil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentinaes_AR
dc.description.filFil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriología e Instituto de Patología; Argentinaes_AR
dc.description.filFil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentinaes_AR
dc.description.filFil: Gimeno, Eduardo Juan. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriología e Instituto de Patología; Argentinaes_AR
dc.description.filFil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentinaes_AR
dc.subtypecientifico


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