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Abstract
Background: The segregation of the hypoblast and the emergence of the pluripotent epiblast mark the final stages of blastocyst formation in mammalian embryos. In bovine embryos the formation of the hypoblast has been partially studied, and evidence shows that MEK signalling plays a limited role in the segregation of this lineage. Here we explored the role of different signalling pathways during lineage segregation in the bovine embryo using [ver mas...]
dc.contributor.authorCanizo, Jésica Romina
dc.contributor.authorYnsaurralde Rivolta, Amanda Eugenia
dc.contributor.authorVazquez Echegaray, Camila
dc.contributor.authorSuvá, Mariana
dc.contributor.authorAlberio, Virgilia
dc.contributor.authorAller Atucha, Juan Florencio
dc.contributor.authorGuberman, Alejandra
dc.contributor.authorSalamone, Daniel
dc.contributor.authorAlberio, Ricardo
dc.contributor.authorAlberio, Ramiro
dc.date.accessioned2019-09-30T11:08:27Z
dc.date.available2019-09-30T11:08:27Z
dc.date.issued2019-07-04
dc.identifier.issn1471-213X
dc.identifier.otherhttps://doi.org/10.1186/s12861-019-0193-9
dc.identifier.urihttps://bmcdevbiol.biomedcentral.com/articles/10.1186/s12861-019-0193-9
dc.identifier.urihttp://hdl.handle.net/20.500.12123/6009
dc.description.abstractBackground: The segregation of the hypoblast and the emergence of the pluripotent epiblast mark the final stages of blastocyst formation in mammalian embryos. In bovine embryos the formation of the hypoblast has been partially studied, and evidence shows that MEK signalling plays a limited role in the segregation of this lineage. Here we explored the role of different signalling pathways during lineage segregation in the bovine embryo using immunofluorescence analysis of NANOG and SOX17 as readouts of epiblast and hypoblast, respectively. Results We show that SOX17 starts to be expressed in 16–32-cell stage embryos, whereas NANOG is first detected from 8-cell stage. SOX17 is first co-expressed with NANOG, but these markers become mutually exclusive by the late blastocyst stage. By assessing the expression kinetics of NANOG/SOX17 we show that inhibition of MEK signalling can eliminate SOX17 expression in bovine blastocysts, without altering NANOG expression. Modulation of WNT, PKC and LIF did not affect NANOG expression in the epiblast when used in combination with the ERK inhibitor. Conclusions This study shows that SOX17 can be used as a reliable early marker of hypoblast in the bovine, and based on its expression profile we show that the hypoblast segregates in day 7 blastocysts. Furthermore, SOX17 expression is abolished using 1 μM of PD0325901, without affecting the NANOG population in the epiblast. Modulation of WNT, PKC and LIF are not sufficient to support enhanced NANOG expression in the epiblast when combined with ERK inhibitor, indicating that additional signalling pathways should be examined to determine their potential roles in epiblast expansion.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherBioMed Centrales_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourceBMC Developmental Biology 19 : 13 (2019)es_AR
dc.subjectGanado Bovinoes_AR
dc.subjectCattleeng
dc.subjectEmbriones Animaleses_AR
dc.subjectAnimal Embryoseng
dc.subjectSegregaciónes_AR
dc.subjectSegregationeng
dc.subjectLinajees_AR
dc.subjectLineageeng
dc.subjectInmunofluorescenciaes_AR
dc.subjectImmunofluorescenceeng
dc.titleA dose-dependent response to MEK inhibition determines hypoblast fate in bovine embryoses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEEA Balcarcees_AR
dc.description.filFil: Canizo, Jesica Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.es_AR
dc.description.filFil: Ynsaurralde Rivolta, Amada Eugenia. Universidad de Buenos Aires. Facultad de Agronomía; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Vazquez Echegaray, Camila. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Suvá, Mariana. Universidad de Buenos Aires. Facultad de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Alberio, Virgilia. Universidad de Buenos Aires. Facultad de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Aller, Juan Florencio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.es_AR
dc.description.filFil: Guberman, Alejandra S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.es_AR
dc.description.filFil: Salamone, Daniel.F. Universidad de Buenos Aires. Facultad de Agronomía; Argentina.es_AR
dc.description.filFil: Alberio, Ricardo H. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.es_AR
dc.description.filFil: Alberio, Ramiro. University of Nottingham. School of Biosciences; Reino Unidoes_AR
dc.subtypecientifico


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