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resumen

Resumen
Rachiplusia nu (Lepidoptera: Noctuidae) is a key soybean pest in Argentina. Current management of this moth relies mainly on the use of synthetic insecticides and transgenic plants. In search of biological control-based alternatives, a baculovirus from R. nu (hereafter RanuNPV) was characterized and its insecticidal properties tested under laboratory conditions. RanuNPV occlusion bodies (OBs) were nearly tetrahedral, averaging 1.0 ± 0.2 μm in their [ver mas...]
dc.contributor.authorJakubowicz, Violeta
dc.contributor.authorTaibo, Catalina Beatriz
dc.contributor.authorSciocco, Alicia Ines
dc.contributor.authorArneodo Larochette, Joel Demian
dc.date.accessioned2019-09-09T17:12:04Z
dc.date.available2019-09-09T17:12:04Z
dc.date.issued2019-09
dc.identifier.issn0022-2011
dc.identifier.otherhttps://doi.org/10.1016/j.jip.2019.107211
dc.identifier.urihttp://hdl.handle.net/20.500.12123/5825
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0022201119300850?via%3Dihub
dc.description.abstractRachiplusia nu (Lepidoptera: Noctuidae) is a key soybean pest in Argentina. Current management of this moth relies mainly on the use of synthetic insecticides and transgenic plants. In search of biological control-based alternatives, a baculovirus from R. nu (hereafter RanuNPV) was characterized and its insecticidal properties tested under laboratory conditions. RanuNPV occlusion bodies (OBs) were nearly tetrahedral, averaging 1.0 ± 0.2 μm in their longest edge and containing singly enveloped nucleocapsids. Histopathology of infected late-instar larvae revealed broad tissue tropism, where fat bodies and epidermis were the most affected organs. Phylogenetic analysis of concatenated polh, lef-8 and lef-9 partial sequences classified RanuNPV as a new species that clusters with other group II alphabaculoviruses infecting larvae of Plusiinae. Bioassays performed with R. nu neonates determined the median lethal dosage to be approximately 2.5 OBs/larva; most insects died within 4–5 days post inoculation showing typical baculovirus-induced liquefaction. No effects were observed in other lepidopteran species assayed, including Spodoptera frugiperda, Cydia pomonella and Diatraea saccharalis. High pathogenicity and host specificity make RanuNPV a good candidate for controlling R. nu.eng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.publisherElsevier
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.sourceJournal of invertebrate pathology 166 : 107211. (September 2019)eng
dc.subjectRachiplusiaes_AR
dc.subjectBaculoviridaees_AR
dc.subjectBeneficial Organismseng
dc.subjectOrganismos Beneficiososes_AR
dc.subjectPathogenesiseng
dc.subjectPatogénesises_AR
dc.subjectPhylogenyeng
dc.subjectFilogeniaes_AR
dc.subjectSoybeanseng
dc.subjectSojaes_AR
dc.subjectGlycine Maxes_AR
dc.subjectBiological Control Agentseng
dc.subjectAgentes de Control Biológicoes_AR
dc.subjectLepidopteraes_AR
dc.subject.otherViral Insecticideeng
dc.titleBiological and molecular characterization of Rachiplusia nu single nucleopolyhedrovirus, a promising biocontrol agent against the South American soybean pest Rachiplusia nueng
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenInstituto de Microbiología y Zoología Agrícola (IMYZA)es_AR
dc.description.filFil: Jakubowicz, Violeta. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de investigaciones Científicas y Tecnológicas. Instituto de Agrobiotecnología y Biología Molecular; Argentinaes_AR
dc.description.filFil: Taibo, Catalina Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas. Servicio de Microscopía; Argentinaes_AR
dc.description.filFil: Sciocco, Alicia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentinaes_AR
dc.description.filFil: Arneodo Larochette, Joel Demian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de investigaciones Científicas y Tecnológicas. Instituto de Agrobiotecnología y Biología Molecular; Argentinaes_AR
dc.subtypecientifico


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