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Abstract
Leptospirosis is a zoonotic disease of global distribution, caused by bacteria of the genus Leptospira. These spirochetes are living organisms free of mud and water; pathogenic leptospires can survive several days in fresh water when pH and temperature are adequate. During 2016, water samples were collected from Callvú Leovú stream (Azul, Buenos Aires); samples were inoculated in liquid EMJH medium and incubated at 28° C for 90 days. Six isolates of [ver mas...]
dc.contributor.authorScialfa, Exequiel
dc.contributor.authorVidela, Yanina
dc.contributor.authorGrune Loffler, Sylvia
dc.contributor.authorQuintana, Silvina
dc.contributor.authorAguirre, Pablo
dc.contributor.authorBrihuega, Bibiana Felicitas
dc.date.accessioned2019-09-05T10:58:22Z
dc.date.available2019-09-05T10:58:22Z
dc.date.issued2019-07
dc.identifier.issn1338-5178
dc.identifier.otherhttps://doi.org/10.15414/jmbfs.2019.8.6.1265-1270
dc.identifier.urihttps://www.jmbfs.org/issue/june-july-2019-vol-8-no-6/jmbfs_77_exequiel/?issue_id=5669&article_id=5
dc.identifier.urihttp://hdl.handle.net/20.500.12123/5782
dc.description.abstractLeptospirosis is a zoonotic disease of global distribution, caused by bacteria of the genus Leptospira. These spirochetes are living organisms free of mud and water; pathogenic leptospires can survive several days in fresh water when pH and temperature are adequate. During 2016, water samples were collected from Callvú Leovú stream (Azul, Buenos Aires); samples were inoculated in liquid EMJH medium and incubated at 28° C for 90 days. Six isolates of saprophytic leptospires and six of spirils (Aquaspirillum spp.) were obtained. The isolates were inoculated in EMJH (liquid and semi-solid) medium and sterile stream water at 4-10° C and 28-30° C; development was observed periodically using dark field microscopy. Both bacteria (alone or together) grew exponentially in first three weeks in all media incubated at 28-30° C; the semi-solid medium was the most efficient at 28-30° C of incubation, and the bacteria remained viable after 16 weeks. At 4-8° C both bacteria remained undetectable but viable in media incubated at 4-8° C for three weeks until the temperature was optimal (thermal stimulation). Leptospires developed in all media used and remained viable for 112 to 168 days (at 4-8° C incubation) in liquid media. The formation of cellular aggregate between Leptospira spp. and Aquaspirillum spp. was independent at the incubation temperature. These results suggest that Aquaspirillum spp. coexists with the genus Leptospira in surface waters, and their presence may indicate possible circulation of leptospires.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherFaculty of Biotechnology and Food Sciences, Slovakiaes_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourceJournal of Microbiology, Biotechnology and Food Sciences 8 (6) : 1265-1270 (Julio 2019)es_AR
dc.subjectLeptospiraes_AR
dc.subjectIsolationeng
dc.subjectAislamientoes_AR
dc.subjectArgentinaes_AR
dc.subjectSaprofitismo
dc.subjectSaprophytismeng
dc.subject.otherAquaspirillumes_AR
dc.titleBiofilm formation by Aquaspirillum spp. and saprophytic Leptospira spp. isolated from environmental source of Argentinees_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Patobiologíaes_AR
dc.description.filFil: Scialfa, Exequiel. Ministerio de Salud. División Zoonosis Rurales; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; Argentinaes_AR
dc.description.filFil: Videla, Yanina. Ministerio de Salud. División Zoonosis Rurales; Argentina. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires; Argentinaes_AR
dc.description.filFil: Grune Loffler, Sylvia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.es_AR
dc.description.filFil: Quintana, Silvina. Instituto de Análisis Fares Taie; Argentinaes_AR
dc.description.filFil: Aguirre, Pablo. Ministerio de Salud. División Zoonosis Rurales; Argentinaes_AR
dc.description.filFil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentinaes_AR
dc.subtypecientifico


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