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Thirty fosmids were randomly selected from a library of Fragaria vesca subsp. americana (cv. Pawtuckaway) DNA. These fosmid clones were individually sheared, and ∼4- to 5-kb fragments were subcloned. Subclones on a single 384-well plate were sequenced bidirectionally for each fosmid. Assembly of these data yielded 12 fosmid inserts completely sequenced, 14 inserts as 2 to 3 contiguous sequences (contigs), and 4 inserts with 5 to 9 contigs. In most cases,
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dc.contributor.author | Pontaroli, Ana Clara | |
dc.contributor.author | Rogers, Rebekah L. | |
dc.contributor.author | Qian, Zhang | |
dc.contributor.author | Shields, Melanie E. | |
dc.contributor.author | Davis, Thomas M. | |
dc.contributor.author | Folta, Kevin M. | |
dc.contributor.author | SanMiguel, Phillip | |
dc.contributor.author | Bennetzen, Jeffrey L. | |
dc.date.accessioned | 2019-07-10T15:29:58Z | |
dc.date.available | 2019-07-10T15:29:58Z | |
dc.date.issued | 2009-03 | |
dc.identifier.issn | 1940-3372 | |
dc.identifier.other | https://doi.org/10.3835/plantgenome2008.09.0007 | |
dc.identifier.uri | https://dl.sciencesocieties.org/publications/tpg/articles/2/1/93 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/5465 | |
dc.description.abstract | Thirty fosmids were randomly selected from a library of Fragaria vesca subsp. americana (cv. Pawtuckaway) DNA. These fosmid clones were individually sheared, and ∼4- to 5-kb fragments were subcloned. Subclones on a single 384-well plate were sequenced bidirectionally for each fosmid. Assembly of these data yielded 12 fosmid inserts completely sequenced, 14 inserts as 2 to 3 contiguous sequences (contigs), and 4 inserts with 5 to 9 contigs. In most cases, a single unambiguous contig order and orientation was determined, so no further finishing was required to identify genes and their relative arrangement. One hundred fifty-eight genes were identified in the ∼1.0 Mb of nuclear genomic DNA that was assembled. Because these fosmids were randomly chosen, this allowed prediction of the genetic content of the entire ∼200 Mb F. vesca genome as about 30,500 protein-encoding genes, plus >4700 truncated gene fragments. The genes are mostly arranged in gene-rich regions, to a variable degree intermixed with transposable elements (TEs). The most abundant TEs in F. vesca were found to be long terminal repeat (LTR) retrotransposons, and these comprised about 13% of the DNA analyzed. Over 30 new repeat families were discovered, mostly TEs, and the total TE content of F. vesca is predicted to be at least 16%. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.publisher | Crop Science Society of America | es_AR |
dc.rights | info:eu-repo/semantics/openAccess | es_AR |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
dc.source | Plant Genome 2 (1) : 93-101 (2009) | es_AR |
dc.subject | Fragaria vesca | es_AR |
dc.subject | Fresa | es_AR |
dc.subject | Genomas | es_AR |
dc.subject | Genomes | eng |
dc.subject | ADN | es_AR |
dc.subject | DNA | eng |
dc.subject | Secuencia Nucleotídica | es_AR |
dc.subject | Nucleotide Sequence | eng |
dc.subject.other | Frutilla de los bosques | es_AR |
dc.subject.other | Woodland Strawberry | eng |
dc.title | Gene content and distribution in the nuclear genome of Fragaria vesca | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
dc.description.origen | EEA Balcarce | es_AR |
dc.description.fil | Fil: Pontaroli, Ana Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. University of Georgia. Department of Genetics; Estados Unidos | es_AR |
dc.description.fil | Fil: Rogers, Rebekah L. Harvard University. Department of Organismic and Evolutionary Biology; Estados Unidos. University of Georgia. Department of Genetics; Estados Unidos | es_AR |
dc.description.fil | Fil: Qian, Zhang. University of New Hampshire. Department of Biological Sciences; Estados Unidos | es_AR |
dc.description.fil | Fil: Shields, Melanie E. University of New Hampshire. Department of Biological Sciences; Estados Unidos | es_AR |
dc.description.fil | Fil: Davis, Thomas M. University of New Hampshire. Department of Biological Sciences; Estados Unidos | es_AR |
dc.description.fil | Fil: Folta, Kevin M. University of Florida. Horticultural Sciences Department; Estados Unidos | es_AR |
dc.description.fil | Fil: SanMiguel, Phillip. Purdue University. Department of Horticulture and Landscape Architecture; Estados Unidos | es_AR |
dc.description.fil | Fil: Bennetzen, Jeffrey L. University of Georgia. Department of Genetics; Estados Unidos | es_AR |
dc.subtype | cientifico |
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