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Abstract
Sheep chromosome 3 (Oar3) has the largest number of QTLs reported to be significantly associated with resistance to gastro-intestinal nematodes. This study aimed to identify single nucleotide polymorphisms (SNPs) within candidate genes located in sheep chromosome 3 as well as genes involved in major immune pathways. A total of 41 SNPs were identified across 38 candidate genes in a panel of unrelated sheep and genotyped in 713 animals belonging to 22 [ver mas...]
dc.contributor.authorPeriasamy, Kathiravan
dc.contributor.authorPichler, Rudolf
dc.contributor.authorPoli, Mario Andres
dc.contributor.authorCristel, Silvina Lujan
dc.contributor.authorCetra, Bibiana Maria
dc.contributor.authorMedus, Pablo Daniel
dc.contributor.authorBasar, Muladno
dc.contributor.authorThiruvenkadan, A.K.
dc.contributor.authorRamasamy, Saravanan
dc.contributor.authorEllahi, Masroor Babbar
dc.contributor.authorMohammed, Faruque
dc.contributor.authorTeneva, Atanaska
dc.contributor.authorShamsuddin, Mohammed
dc.contributor.authorGarcia Podesta, Mario
dc.contributor.authorDiallo, Adama
dc.date.accessioned2019-06-11T13:17:16Z
dc.date.available2019-06-11T13:17:16Z
dc.date.issued2014-02-12
dc.identifier.issn1932-6203
dc.identifier.otherhttps://doi.org/10.1371/journal.pone.0088337
dc.identifier.urihttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0088337
dc.identifier.urihttp://hdl.handle.net/20.500.12123/5295
dc.description.abstractSheep chromosome 3 (Oar3) has the largest number of QTLs reported to be significantly associated with resistance to gastro-intestinal nematodes. This study aimed to identify single nucleotide polymorphisms (SNPs) within candidate genes located in sheep chromosome 3 as well as genes involved in major immune pathways. A total of 41 SNPs were identified across 38 candidate genes in a panel of unrelated sheep and genotyped in 713 animals belonging to 22 breeds across Asia, Europe and South America. The variations and evolution of immune pathway genes were assessed in sheep populations across these macro-environmental regions that significantly differ in the diversity and load of pathogens. The mean minor allele frequency (MAF) did not vary between Asian and European sheep reflecting the absence of ascertainment bias. Phylogenetic analysis revealed two major clusters with most of South Asian, South East Asian and South West Asian breeds clustering together while European and South American sheep breeds clustered together distinctly. Analysis of molecular variance revealed strong phylogeographic structure at loci located in immune pathway genes, unlike microsatellite and genome wide SNP markers. To understand the influence of natural selection processes, SNP loci located in chromosome 3 were utilized to reconstruct haplotypes, the diversity of which showed significant deviations from selective neutrality. Reduced Median network of reconstructed haplotypes showed balancing selection in force at these loci. Preliminary association of SNP genotypes with phenotypes recorded 42 days post challenge revealed significant differences (P<0.05) in fecal egg count, body weight change and packed cell volume at two, four and six SNP loci respectively. In conclusion, the present study reports strong phylogeographic structure and balancing selection operating at SNP loci located within immune pathway genes. Further, SNP loci identified in the study were found to have potential for future large scale association studies in naturally exposed sheep populations.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherPlos Onees_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourcePLoS ONE 9 (2) : e88337. (February 2014)es_AR
dc.subjectOvinoses_AR
dc.subjectSheepeng
dc.subjectParásitoses_AR
dc.subjectParasiteseng
dc.subjectGenéticaes_AR
dc.subjectGeneticseng
dc.subjectResistencia a las Plagases_AR
dc.subjectPest Resistanceeng
dc.subjectRespuesta Inmunológicaes_AR
dc.subjectImmune Responseeng
dc.subjectLoci de Rasgos Cuantitativoses_AR
dc.subjectQuantitative Trait Locieng
dc.subjectcientifico
dc.subject.otherQTLes_AR
dc.titleCandidate Gene Approach for Parasite Resistance in Sheep – Variation in Immune Pathway Genes and Association with Fecal Egg Countes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Genéticaes_AR
dc.description.filFil: Periasamy, Kathiravan. Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency. Animal Production and Health Laboratory; Austriaes_AR
dc.description.filFil: Pichler, Rudolf. Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency. Animal Production and Health Laboratory; Austriaes_AR
dc.description.filFil: Poli, Mario Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentinaes_AR
dc.description.filFil: Cristel, Silvina Lujan. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentinaes_AR
dc.description.filFil: Cetra, Bibiana Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentinaes_AR
dc.description.filFil: Medus, Pablo Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentinaes_AR
dc.description.filFil: Basar, Muladno. Bogor Agricultural University. Department of Animal Sciences; Indonesiaes_AR
dc.description.filFil: Thiruvenkadan, A.K. Tamil Nadu Veterinary and Animal Sciences University. Veterinary College and Research Institute-Namakkal; Indiaes_AR
dc.description.filFil: Ramasamy, Saravanan. Tamil Nadu Veterinary and Animal Sciences University. Veterinary College and Research Institute-Namakkal; Indiaes_AR
dc.description.filFil: Ellahi, Masroor Babbar. University of Veterinary and Animal Sciences. Department of Animal Genetics and Breeding; Paquistánes_AR
dc.description.filFil: Mohammed, Faruque. Bangladesh Agricultural University. Department of Animal Breeding and Genetics; Bangladeshes_AR
dc.description.filFil: Teneva, Atanaska. University of Forestry; Bulgariaes_AR
dc.description.filFil: Shamsuddin, Mohammed. Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency. Animal Production and Health Laboratory; Austriaes_AR
dc.description.filFil: Garcia Podesta, Mario. Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency. Animal Production and Health Laboratory; Austriaes_AR
dc.description.filFil: Diallo, Adama. Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency. Animal Production and Health Laboratory; Austriaes_AR


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