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Abstract
Antivenom for the treatment of bothropic snakebite is a priority for public health institutions from Latin America. An alternative to the conventional antivenom production is based on the use of egg yolk antibodies – IgY-technology – by immunizing laying hens. In this study, we produced, characterized and assessed the efficacy of IgY-based antivenoms against B. alternatus venom. Immunochemical studies (reactivity, avidity and antigen recognition pattern) [ver mas...]
dc.contributor.authorLeiva, Carlos Leónidas
dc.contributor.authorCangelosi, Adriana
dc.contributor.authorMariconda, Virginia
dc.contributor.authorFarace, Mariano
dc.contributor.authorGeoghegan, Patricia
dc.contributor.authorBrero, Luisa
dc.contributor.authorFernandez Miyakawa, Mariano Enrique
dc.contributor.authorChacana, Pablo Anibal
dc.date.accessioned2019-05-17T13:07:57Z
dc.date.available2019-05-17T13:07:57Z
dc.date.issued2019-05
dc.identifier.issn0041-0101
dc.identifier.otherhttps://doi.org/10.1016/j.toxicon.2019.03.020
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0041010119300972
dc.identifier.urihttp://hdl.handle.net/20.500.12123/5141
dc.description.abstractAntivenom for the treatment of bothropic snakebite is a priority for public health institutions from Latin America. An alternative to the conventional antivenom production is based on the use of egg yolk antibodies – IgY-technology – by immunizing laying hens. In this study, we produced, characterized and assessed the efficacy of IgY-based antivenoms against B. alternatus venom. Immunochemical studies (reactivity, avidity and antigen recognition pattern) as well as antivenom efficacy assays were performed. After the 3rd immunization, levels of specific IgY reached a maximum that was maintained throughout the observation period, while avidity indexes of the extracts increased after the successive immunizations. Furthermore, IgY against B. alternatus recognized protein complexes of the venom with high (>40 kDa), medium (20–40 kDa) and low (<20 kDa) molecular weights. IgY antivenoms obtained after 8 immunizations neutralized 35.65 μg of B. alternatus venom per mg of antivenom, while specific activities values ranged from 0.28 to 0.42. In conclusion, we produced and characterized IgY antivenoms capable of neutralizing the lethal activity of B. alternatus venom at a preclinical level. Thus, IgY-technology may allow the production of effective and affordable antivenoms fulfilling the urgent needs of many countries where conventional manufacture is unable to provide enough availability of antivenoms.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherElsevieres_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceToxicon 163 : 84-92 (Mayo 2019)es_AR
dc.subjectEgg Yolkeng
dc.subjectYema de Huevoes_AR
dc.subjectSnake Biteseng
dc.subjectMordeduras de Serpienteses_AR
dc.subjectVenomseng
dc.subjectVenenoses_AR
dc.subjectAntígenos
dc.subjectAntigenseng
dc.subject.otherBothrops alternatuses_AR
dc.subject.otherSnake Venomeng
dc.subject.otherVeneno de Serpientees_AR
dc.subject.otherIgY Antibodyeng
dc.subject.otherAnticuerpo IgYes_AR
dc.subject.otherVenom Neutralizationeng
dc.subject.otherNeutralización del Venenoes_AR
dc.titleIgY-based antivenom against Bothrops alternatus: Production and neutralization efficacyes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Patobiologíaes_AR
dc.description.filFil: Leiva, Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Cangelosi, Adriana. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentinaes_AR
dc.description.filFil: Mariconda, Virginia. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentinaes_AR
dc.description.filFil: Farace, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Geoghegan, Patricia. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentinaes_AR
dc.description.filFil: Brero, Luisa. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentinaes_AR
dc.description.filFil: Fernandez Miyakawa, Mariano Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentinaes_AR
dc.subtypecientifico


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