Show simple item record

resumen

Abstract
A sequence‐based approach was used to investigate molecular genetic variations in Phakopsora pachyrhizi, an obligate biotrophic pathogen that causes Asian soybean rust. In Argentina, the samples came from uredinium‐bearing leaves taken from 11 soybean fields; in Brazil, the samples comprised urediniospores from leaves of 10 soybean genotypes that had been grown in three experimental stations during two growing seasons. PCR‐based cloning techniques were [ver mas...]
dc.contributor.authorJorge, V.R.
dc.contributor.authorSilva, M.R.
dc.contributor.authorGuillin, Eduardo Alejandro
dc.contributor.authorFreire, M.C.M.
dc.contributor.authorSchuster, I.
dc.contributor.authorAlmeida, A.M.R.
dc.contributor.authorOliveira, Luiz O.
dc.date.accessioned2019-05-17T13:03:49Z
dc.date.available2019-05-17T13:03:49Z
dc.date.issued2015-06
dc.identifier.issn0032-0862
dc.identifier.issn1365-3059
dc.identifier.otherhttps://doi.org/10.1111/ppa.12300
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/full/10.1111/ppa.12300
dc.identifier.urihttp://hdl.handle.net/20.500.12123/5140
dc.description.abstractA sequence‐based approach was used to investigate molecular genetic variations in Phakopsora pachyrhizi, an obligate biotrophic pathogen that causes Asian soybean rust. In Argentina, the samples came from uredinium‐bearing leaves taken from 11 soybean fields; in Brazil, the samples comprised urediniospores from leaves of 10 soybean genotypes that had been grown in three experimental stations during two growing seasons. PCR‐based cloning techniques were used to generate DNA sequences for two gene regions and alignments were supplemented with data from GenBank. A total of 575 sequences for the internal transcribed spacer region (18 ribotypes) and 160 partial sequences for a housekeeping gene encoding ADP‐ribosylation factor (10 haplotypes) were obtained. Ribotype accumulation curves predicted that about 20 bacterial clones would recover 5–6 ribotypes (c. 70–80% of the total molecular variation) per locality. The samples from the three experimental stations in Brazil displayed most (14 out of 16) ribotypes found worldwide; the lack of genetic structure and differentiation at a diverse geographic scale suggests that both local and distant sources provide airborne inoculum during disease establishment. Soybean genotypes with resistance genes for the Asian soybean rust did not decrease the molecular genetic variation of fungal populations.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherWileyes_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourcePlant Pathology 64 (3) : 729-737 (June 2015)es_AR
dc.subjectSojaes_AR
dc.subjectSoybeanseng
dc.subjectEnfermedades de las Plantases_AR
dc.subjectPlant Diseaseseng
dc.subjectRoyaes_AR
dc.subjectRustseng
dc.subjectPhakopsora pachyrhizies_AR
dc.subjectVariación Genéticaes_AR
dc.subjectGenetic Variationeng
dc.subjectAmérica del Sures_AR
dc.subjectSouth Americaeng
dc.subject.otherRoya de la Sojaes_AR
dc.subject.otherRoya Asiáticaes_AR
dc.subject.otherDiversidad Genéticaes_AR
dc.titleThe origin and genetic diversity of the causal agent of Asian soybean rust, Phakopsora pachyrhizi, in South Americaes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Genéticaes_AR
dc.description.filFil: Jorge, V.R. Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular; Brasiles_AR
dc.description.filFil: Silva, M.R. Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular; Brasiles_AR
dc.description.filFil: Guillin, Eduardo Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentinaes_AR
dc.description.filFil: Freire, M.C.M. Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular; Brasiles_AR
dc.description.filFil: Schuster, I. Cooperativa Central de Pesquisa Agrícola; Brasiles_AR
dc.description.filFil: Almeida, A.M.R. Empresa Brasileira de Pesquisa Agropecuária, Embrapa‐Soja; Brasiles_AR
dc.description.filFil: Oliveira, Luiz O. Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular; Brasiles_AR
dc.subtypecientifico


Files in this item

Thumbnail

This item appears in the following Collection(s)

common

Show simple item record