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Abstract
Several hydrogel surfaces present properties that simulate the mechanical and physicochemical features of extracellular matrix (ECM), providing a platform that mimic the native cellular milieus. Poly-N-isopropylacrylamide (PNIPAM) hydrogels are receiving attention in biomedical field due to their thermosensibility and soft texture. However, more extensive biocompatibility and cellular interactions studies with cell lines are needed. Therefore, the aim of [ver mas...]
dc.contributor.authorCapella, Virginia
dc.contributor.authorRivero, Rebeca E.
dc.contributor.authorLiaudat, Ana Cecilia
dc.contributor.authorIbarra, Luis E.
dc.contributor.authorRoma, Dardo Andrés
dc.contributor.authorAlustiza, Fabrisio Eduardo
dc.contributor.authorMañas, Fernando
dc.contributor.authorBarbero, César A.
dc.contributor.authorBosch, Pablo
dc.contributor.authorRivarola, Claudia R.
dc.contributor.authorRodríguez, Nancy
dc.date.accessioned2019-05-02T18:16:52Z
dc.date.available2019-05-02T18:16:52Z
dc.date.issued2019-04-09
dc.identifier.issn2405-8440
dc.identifier.otherhttps://doi.org/10.1016/j.heliyon.2019.e01474
dc.identifier.urihttps://www.heliyon.com/article/e01474/
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S2405844018387383
dc.identifier.urihttp://hdl.handle.net/20.500.12123/5020
dc.description.abstractSeveral hydrogel surfaces present properties that simulate the mechanical and physicochemical features of extracellular matrix (ECM), providing a platform that mimic the native cellular milieus. Poly-N-isopropylacrylamide (PNIPAM) hydrogels are receiving attention in biomedical field due to their thermosensibility and soft texture. However, more extensive biocompatibility and cellular interactions studies with cell lines are needed. Therefore, the aim of this study is focus on evaluating the biocompatibility of PNIPAM through cytotoxicity, genotoxicity, and proliferation tests in murine preadipose cells (3T3-L1), human embryonic kidney cells (HEK293) and human carcinoma-derived cells (A549) in presence of hydrogel surfaces. Bioadhesive capacity above PNIPAM surfaces was also analyzed. MTT and neutral red uptake assays shown non-cytotoxic effect of PNIPAM in the studied cell lines. Genotoxicity was evaluated by the single-cell gel electrophoresis assay, where DNA damages were not detected. [3H]-thymidine staining allowed to corroborate that cell proliferation had progressed correctly. Adopted morphologies for each cell line over PNIPAM were similar to cell growing observed on polystyrene, indicating that the surfaces favor the cell attachment during 5 days' culture. The good biocompatibility of PNIPAM surfaces make it an interesting scaffold with clinical potential in tissue regeneration engineering, and a possible adipose and kidney tissue-engineered construct.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherElsevieres_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourceHeliyon 5 (4) : e01474 (April 2019)es_AR
dc.subjectCitotoxicidades_AR
dc.subjectCytotoxicityeng
dc.subjectBiotecnologíaes_AR
dc.subjectBiotechnologyeng
dc.subjectCélulases_AR
dc.subjectCellseng
dc.subjectADNes_AR
dc.subjectDNAeng
dc.subject.otherHidrogeleses_AR
dc.subject.otherBioingenieríaes_AR
dc.titleCytotoxicity and bioadhesive properties of poly-N-isopropylacrylamide hydrogeles_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEEA Marcos Juárezes_AR
dc.description.filFil: Capella, Virginia. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Rivero, Rebeca E. Universidad Nacional de Río Cuarto. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Liaudat, Ana Cecilia. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina.Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Ibarra, Luis E. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina.Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Roma, Dardo Andrés. Universidad Nacional de Río Cuarto. Departamento de Clínica Animal; Argentinaes_AR
dc.description.filFil: Alustiza, Fabrisio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentinaes_AR
dc.description.filFil: Mañas, Fernando. Universidad Nacional de Río Cuarto. Departamento de Clínica Animal; Argentinaes_AR
dc.description.filFil: Barbero, César A. Universidad Nacional de Río Cuarto. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Bosch, Pablo. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Rivarola, Claudia R. Universidad Nacional de Río Cuarto. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Rodríguez, Nancy. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.subtypecientifico


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