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An isopentenyl transferase transgenic wheat isoline exhibits less seminal root growth impairment and a differential metabolite profile under Cd stress
Gomez Mansur, Nabila M.; Pena, Liliana Beatriz; Bossio, Adrian Ezequiel; Lewi, Dalia Marcela; Beznec, Ailin; Blumwald, Eduardo; Arbona, Vicent; Gómez-Cadenas, Aurelio; Benavides, María Patricia; Gallego, Susana Mabel (Wiley, 2021-09)Cadmium is one of the most important contaminants and it induces severe plant growth restriction. In this study, we analyzed the metabolic changes associated with root growth restriction caused by cadmium in the early ... -
CRISPR/Cas9-mediated knockout of a polyester synthase-like gene delays flowering time in alfalfa
Galindo Sotomonte, Luisa Fernanda; Jozefkowicz, Cintia; Gomez, Maria Cristina; Stritzler, Margarita; Frare, Romina Alejandra; Bottero, Ana Emilia; Tajima, Hiromi; Blumwald, Eduardo; Ayub, Nicolás Daniel; Soto, Gabriela Cynthia (Springer, 2023-02)Alfalfa (Medicago sativa), commonly known as the “Queen of Forages”, is the main source of plant protein for meat and milk production systems worldwide. Since alfalfa is a perennial vegetative-harvest forage, delaying ... -
Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought
Beznec, Ailin; Faccio, Paula Daniela; Miralles, Daniel J.; Abeledo, Leonor Gabriela; Decima Oneto, Cecilia Andrea; Garibotto, María De Belén; Gonzalez, Germán Andrés; Moreyra, Federico; Elizondo, Matias; Ruiz, Monica Beatriz; Lewi, Dalia Marcela; Blumwald, Eduardo; Llorente, Berta; Diaz Paleo, Antonio Horacio; Bossio, Adrian Ezequiel (Springer, 2021-05)Background: The heterologous expression of isopentenyl transferase (IPT) under the transcriptional control of the senescence-associated receptor-like kinase (SARK) promoter delayed cellular senescence and, through it, ... -
Validation of housekeeping genes for qPCR in maize during water deficit stress conditions at flowering time
Decima Oneto, Cecilia Andrea; Bossio, Adrian Ezequiel; Faccio, Paula Daniela; Beznec, Ailin; Blumwald, Eduardo; Lewi, Dalia Marcela (2017)Plant stress studies are increasingly being based on gene expression. The analysis of gene expression requires sensitive, precise, and replicable measurements for specific mRNA sequences. Real-time RT-PCR is nowadays the ... -
Water deficit stress tolerance in maize conferred by expression of an anisopentenyltransferase (IPT) gene driven by a stress- and maturation-induced promoter
Decima Oneto, Cecilia Andrea; Otegui, María Elena; Baroli, Irene; Beznec, Ailin; Faccio, Paula Daniela; Bossio, Adrian Ezequiel; Blumwald, Eduardo; Lewi, Dalia Marcela (2016)Senescence can be delayed in transgenic plants overexpressing the enzyme isopentenyltransferase (IPT) due to stress-induced increased levels of endogenous cytokinins. This trait leads to sustained photo-synthetic activity ...