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Resumen
Reproductive biotechnologies such as in vitro fertilization (IVF) and somatic cell nuclear transfer (SCNT) enable improved reproductive efficiency of animals. However, the birth rate of in vitro-derived embryos still lags behind that of their in vivo counterparts. Thus, it is critical to develop an accurate evaluation and prediction system of embryo competence, both for commercial purposes and for scientific research. Previous works have demonstrated that [ver mas...]
dc.contributor.authorCánepa, María Jesús
dc.contributor.authorOrtega, Nicolás Matías
dc.contributor.authorMonteleone, Melisa Carolina
dc.contributor.authorMucci, Nicolas Crescencio
dc.contributor.authorKaiser, German Gustavo
dc.contributor.authorBrocco, Marcela Adriana
dc.contributor.authorMutto, Adrián Angel
dc.date.accessioned2019-04-11T13:24:19Z
dc.date.available2019-04-11T13:24:19Z
dc.date.issued2014-09
dc.identifier.issn1932-6203
dc.identifier.otherhttps://doi.org/10.1371/journal.pone.0108139
dc.identifier.urihttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0108139
dc.identifier.urihttp://hdl.handle.net/20.500.12123/4880
dc.description.abstractReproductive biotechnologies such as in vitro fertilization (IVF) and somatic cell nuclear transfer (SCNT) enable improved reproductive efficiency of animals. However, the birth rate of in vitro-derived embryos still lags behind that of their in vivo counterparts. Thus, it is critical to develop an accurate evaluation and prediction system of embryo competence, both for commercial purposes and for scientific research. Previous works have demonstrated that in vitro culture systems induce alterations in the relative abundance (RA) of diverse transcripts and thus compromise embryo quality. The aim of this work was to analyze the RA of a set of genes involved in cellular stress (heat shock protein 70-kDa, HSP70), endoplasmic reticulum (ER) stress (immunoglobulin heavy chain binding protein, Bip; proteasome subunit β5, PSMB5) and apoptosis (BCL-2 associated X protein, Bax; cysteine aspartate protease-3, Caspase-3) in bovine blastocysts produced by IVF or SCNT and compare it with that of their in vivo counterparts. Poly (A) + mRNA was isolated from three pools of 10 blastocysts per treatment and analyzed by real-time RT-PCR. The RA of three of the stress indicators analyzed (Bax, PSMB5 and Bip) was significantly increased in SCNT embryos as compared with that of in vivo-derived blastocysts. No significant differences were found in the RA of HSP70 and Caspase-3 gene transcripts. This study could potentially complement morphological analyses in the development of an effective and accurate technique for the diagnosis of embryo quality, ultimately aiding to improve the efficiency of assisted reproductive techniques (ART).eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherPlos Onees_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.sourcePLoS ONE 9 (9) : e108139 (September 2014)es_AR
dc.subjectBiotecnologíaes_AR
dc.subjectBiotechnologyeng
dc.subjectReproducciónes_AR
dc.subjectReproductioneng
dc.subjectBiotecnología Animales_AR
dc.subjectAnimal Biotechnologyeng
dc.subjectDiferenciación Celulares_AR
dc.subjectCell Differentiationeng
dc.subjectTransferencia de Embrioneses_AR
dc.subjectEmbryo Transfereng
dc.subjectGanado Bovinoes_AR
dc.subjectCattleeng
dc.subjectFecundación in Vitroes_AR
dc.subjectIn Vitro Fertilizationeng
dc.subjectGeneses_AR
dc.titleExpression profile of genes as indicators of developmental competence and quality of in vitro fertilization and somatic cell nuclear transfer bovine embryoses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.rights.licenseCreative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.description.origenEEA Balcarcees_AR
dc.description.filFil: Cánepa, María Jesús. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentinaes_AR
dc.description.filFil: Ortega, Nicolás Matías. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentinaes_AR
dc.description.filFil: Monteleone, Melisa Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentinaes_AR
dc.description.filFil: Mucci, Nicolas Crescencio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Biotecnología de la Reproducción; Argentinaes_AR
dc.description.filFil: Kaiser, German Gustavo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Biotecnología de la Reproducción; Argentinaes_AR
dc.description.filFil: Brocco, Marcela Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentinaes_AR
dc.description.filFil: Mutto, Adrián Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentinaes_AR
dc.subtypecientifico


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