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Abstract
The primary and secondary structures of the intergenic spacer (IGS) between the 3′-end of 25S ribosomal RNA (rRNA) gene and the 5′-end of 18S rRNA gene are described for the cultivated chili pepper Capsicum pubescens. The recognized functional IGS is 2,078 bp in length. According to nucleotide base composition, regulatory elements, and conserved and repeated sequences the IGS can be divided into seven structural regions (SRI–VII). SRI comprises three [ver mas...]
dc.contributor.authorGrabiele, Mauro
dc.contributor.authorDebat, Humberto Julio
dc.contributor.authorMoscone, Eduardo Alberto
dc.contributor.authorDucasse, Daniel Adrian
dc.date.accessioned2019-04-09T14:26:00Z
dc.date.available2019-04-09T14:26:00Z
dc.date.issued2012-02
dc.identifier.issn0378-2697
dc.identifier.issn1615-6110
dc.identifier.otherhttps://doi.org/10.1007/s00606-011-0546-8
dc.identifier.urihttps://link.springer.com/article/10.1007/s00606-011-0546-8
dc.identifier.urihttp://hdl.handle.net/20.500.12123/4859
dc.description.abstractThe primary and secondary structures of the intergenic spacer (IGS) between the 3′-end of 25S ribosomal RNA (rRNA) gene and the 5′-end of 18S rRNA gene are described for the cultivated chili pepper Capsicum pubescens. The recognized functional IGS is 2,078 bp in length. According to nucleotide base composition, regulatory elements, and conserved and repeated sequences the IGS can be divided into seven structural regions (SRI–VII). SRI comprises three copies of GAGGTTTTT-like motif, a probable transcription termination site in Solanaceae. At 3′-end, there are 21 bp matching the 18S rDNA. SRII is formed by 47 repeats of CACCATGG-like motif, the shortest repetitive region found in plant rDNA to date. SRIII is highly AT-rich, preceding SRIV, a highly conserved region in Solanaceae containing the transcription initiation site (TIS) TATATAAGGGGGG. The external transcribed spacer (ETS) is 966 bp in length. SRV-VII, downstream of the TIS, possesses eight inverted repeats, and three predicted stem-loops show pre-micro RNA (miRNA)-like structural features. Intragenomic variation is presented, and data are compared with characterized Solanaceae 25S–18S rDNA IGS.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherSpringeres_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourcePlant Systematics and Evolution 298 (2) : 313–321 (February 2012)es_AR
dc.subjectCapsicumes_AR
dc.subjectGenéticaes_AR
dc.subjectGeneticseng
dc.subjectADNes_AR
dc.subjectDNAeng
dc.subjectADN Intergénicoes_AR
dc.subjectIntergenic DNAeng
dc.subjectMarcadores Genéticoses_AR
dc.subjectGenetic Markerseng
dc.subject.otherMarcadores Moleculareses_AR
dc.title25S–18S rDNA IGS of Capsicum: molecular structure and comparisones_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Patología Vegetales_AR
dc.description.filFil: Grabiele, Mauro. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentinaes_AR
dc.description.filFil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentinaes_AR
dc.description.filFil: Moscone, Eduardo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentinaes_AR
dc.description.filFil: Ducasse, Daniel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentinaes_AR
dc.subtypecientifico


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