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Abstract
Intoxication with Solanum bonariense in cattle causes cerebellar cortical degeneration with perikaryal vacuolation, axonal swelling, and death primarily of Purkinje cells, with accumulation of electron-dense residual storage bodies in membrane-bound vesicles. The pathogenesis of this disease is not fully understood. Previously, we proposed that inhibition of protein synthesis in Purkinje cells among other altered metabolic pathways could lead to [ver mas...]
dc.contributor.authorVerdes, José Manuel
dc.contributor.authorMárquez, Mercedes
dc.contributor.authorCalliari, Aldo
dc.contributor.authorBattes, Daniel
dc.contributor.authorMoraña, José Antonio
dc.contributor.authorGimeno, Eduardo Juan
dc.contributor.authorOdriozola, Ernesto Raul
dc.contributor.authorGiannitti, Federico
dc.contributor.authorGuerrero, Florentina
dc.contributor.authorFidalgo, Luis Eusebio
dc.contributor.authorPumarola, Martí
dc.date.accessioned2019-04-08T11:48:36Z
dc.date.available2019-04-08T11:48:36Z
dc.date.issued2015-05
dc.identifier.issn1040-6387
dc.identifier.issn1943-4936
dc.identifier.otherhttps://doi.org/10.1177/1040638715582048
dc.identifier.urihttps://journals.sagepub.com/doi/10.1177/1040638715582048
dc.identifier.urihttp://hdl.handle.net/20.500.12123/4834
dc.description.abstractIntoxication with Solanum bonariense in cattle causes cerebellar cortical degeneration with perikaryal vacuolation, axonal swelling, and death primarily of Purkinje cells, with accumulation of electron-dense residual storage bodies in membrane-bound vesicles. The pathogenesis of this disease is not fully understood. Previously, we proposed that inhibition of protein synthesis in Purkinje cells among other altered metabolic pathways could lead to cytoskeletal alterations, subsequently altering cell-specific axonal transport. In the present study, immunohistochemical and histochemical methods were used to identify neuronal cytoskeletal alterations and axonal loss, demyelination, and astrogliosis in the cerebellum of intoxicated bovines. Samples of cerebellum from 3 natural and 4 experimental cases and 2 control bovines were studied. Immunoreactivity against neurofilament (NF)-200KDa confirmed marked loss of Purkinje neurons, and phospho-NF protein, β-tubulin, and affinity reaction against phalloidin revealed an altered perikaryal distribution of neuronal cytoskeletal proteins in the remaining Purkinje cells in intoxicated cattle. Reactive astrogliosis in every layer of the cerebellar cortex was also observed with anti–glial fibrillary acidic protein immunohistochemistry. In affected cattle, demyelination and axonal loss in the cerebellar white matter, as well as basket cell loss were demonstrated with Klüver–Barrera and Bielschowsky stains, respectively. Based on these results, we propose that neuronal cytoskeletal alterations with subsequent interference of the axonal transport in Purkinje cells may play a relevant role in the pathogenesis of this neurodegenerative disorder, and also that demyelination and axonal loss in the cerebellar white matter, as well as astrogliosis in the gray matter, likely occur secondarily to Purkinje cell degeneration and death.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherAmerican Association of Veterinary Laboratory Diagnosticianses_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceJournal of Veterinary Diagnostic Investigation 27 (3) : 278-286 (May 2015)es_AR
dc.subjectGanado Bovinoes_AR
dc.subjectCattleeng
dc.subjectEnfermedades de los Animaleses_AR
dc.subjectAnimal Diseaseseng
dc.subjectEnvenenamientoes_AR
dc.subjectPoisoningeng
dc.subjectSolanumes_AR
dc.subject.otherIntoxicaciónes_AR
dc.subject.otherSolanum bonariensees_AR
dc.titleA novel pathogenic mechanism for cerebellar lesions produced by Solanum bonariense in cattlees_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEEA Balcarcees_AR
dc.description.filFil: Verdes, José Manuel. Universidad de la República. Facultad de Veterinaria; Uruguay. Instituto de Investigaciones Biológicas Clemente Estable; Uruguayes_AR
dc.description.filFil: Márquez, Mercedes. Universitat Autònoma de Barcelona. Veterinary Faculty. Department of Animal Medicine and Surgery; Españaes_AR
dc.description.filFil: Calliari, Aldo. Universidad de la República. Facultad de Veterinaria; Uruguay. Instituto de Investigaciones Biológicas Clemente Estable; Uruguayes_AR
dc.description.filFil: Battes, Daniel. Universidad de la República. Facultad de Veterinaria; Uruguay.es_AR
dc.description.filFil: Moraña, José Antonio. Universidad de la República. Facultad de Veterinaria; Uruguay.es_AR
dc.description.filFil: Gimeno, Eduardo Juan. Universidad Nacional de La Plata, Facultad de Ciencias Veterinarias; Argentinaes_AR
dc.description.filFil: Odriozola, Ernesto Raul. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentinaes_AR
dc.description.filFil: Giannitti, Federico. Instituto Nacional de Investigaciones Agropecuarias. La Estanzuela, Colonia; Uruguayes_AR
dc.description.filFil: Guerrero, Florentina. Universidad de Santiago de Compostela. Facultad de Veterinaria. Departments of Anatomy and Animal Production; Españaes_AR
dc.description.filFil: Fidalgo, Luis Eusebio. Universidad de Santiago de Compostela. Facultad de Veterinaria. Veterinary Clinical Sciences; Españaes_AR
dc.description.filFil: Pumarola, Martí. Universitat Autònoma de Barcelona. Veterinary Faculty. Department of Animal Medicine and Surgery; Españaes_AR
dc.subtypecientifico


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