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Abstract
The vast majority of Pseudomonas species are unable to fix atmospheric nitrogen. Although several studies have demonstrated that some strains belonging to the genus Pseudomonas sensu stricto do have the ability to fix nitrogen by the expression of horizontally acquired nitrogenase, little is known about the mechanisms of nitrogenase adaptation to the new bacterial host. Recently, we transferred the nitrogen fixation island from Pseudomonas stutzeri A1501 [ver mas...]
dc.contributor.authorPascuan, Cecilia Gabriela
dc.contributor.authorFox, Ana Romina
dc.contributor.authorSoto, Gabriela Cinthia
dc.contributor.authorAyub, Nicolás Daniel
dc.date.accessioned2019-04-01T12:47:21Z
dc.date.available2019-04-01T12:47:21Z
dc.date.issued2015-10
dc.identifier.issn0022-2844
dc.identifier.issn1432-1432 (Online)
dc.identifier.other10.1007/s00239-015-9698-4
dc.identifier.urihttp://hdl.handle.net/20.500.12123/4793
dc.identifier.urihttps://link.springer.com/article/10.1007/s00239-015-9698-4
dc.description.abstractThe vast majority of Pseudomonas species are unable to fix atmospheric nitrogen. Although several studies have demonstrated that some strains belonging to the genus Pseudomonas sensu stricto do have the ability to fix nitrogen by the expression of horizontally acquired nitrogenase, little is known about the mechanisms of nitrogenase adaptation to the new bacterial host. Recently, we transferred the nitrogen fixation island from Pseudomonas stutzeri A1501 to the non-nitrogen-fixing bacterium Pseudomonas protegens Pf-5, and interestingly, the resulting recombinant strain Pf-5 X940 showed an uncommon phenotype of constitutive nitrogenase activity. Here, we integrated evolutionary and functional approaches to elucidate this unusual phenotype. Phylogenetic analysis showed that polyhydroxybutyrate (PHB) biosynthesis genes from natural nitrogen-fixing Pseudomonas strains have been acquired by horizontal transfer. Contrary to Pf-5 X940, its derived PHB-producing strain Pf-5 X940-PHB exhibited the inhibition of nitrogenase activity under nitrogen-excess conditions, and displayed the typical switch-on phenotype observed in natural nitrogen-fixing strains after nitrogen deficiency. This indicates a competition between PHB production and nitrogen fixation. Therefore, we propose that horizontal transfer of PHB biosynthesis genes could be an ancestral mechanism of regulation of horizontally acquired nitrogenases in the genus Pseudomonas.eng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.publisherSpringer
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.sourceJournal of molecular evolution 81 (3–4) : 84–89. (October 2015)eng
dc.subjectNitrogenasaes_AR
dc.subjectNitrogenaseeng
dc.subjectEvolutioneng
dc.subjectEvoluciónes_AR
dc.subjectPseudomonases_AR
dc.subjectTransferencia de Genes
dc.subjectGene Transfereng
dc.subject.otherPolyhydroxybutyrateeng
dc.subject.otherHorizontal Transfereng
dc.titleExploring the ancestral mechanisms of regulation of horizontally acquired nitrogenaseseng
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenInstituto de Genéticaes_AR
dc.description.filFil: Pascuan, Cecilia Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Fox, Ana Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.subtypecientifico


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