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Abstract
In this study, we investigated the protective ability of the addition of two antioxidant herb extracts, mate tea and lemon balm, on boar epididymal frozen–thawed spermatozoa quality. Testes from mature boars were collected at local slaughterhouse, and sperm samples from epididymis were recovered by flushing. Spermatozoa were cryopreserved in lactose–egg yolk buffer supplemented with various concentrations of lemon balm and mate tea (0, 2.5, 5 and 10 g [ver mas...]
dc.contributor.authorLuño, Victoria
dc.contributor.authorGil, María Lydia
dc.contributor.authorOlaciregui, Maite
dc.contributor.authorJerez, Rodrigo A.
dc.contributor.authorDe Blas, José Ignacio
dc.contributor.authorHozbor, Federico Andres
dc.date.accessioned2019-03-26T12:11:57Z
dc.date.available2019-03-26T12:11:57Z
dc.date.issued2015-11
dc.identifier.issn0303-4569
dc.identifier.issn1439-0272
dc.identifier.otherhttps://doi.org/10.1111/and.12370
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/abs/10.1111/and.12370
dc.identifier.urihttp://hdl.handle.net/20.500.12123/4737
dc.description.abstractIn this study, we investigated the protective ability of the addition of two antioxidant herb extracts, mate tea and lemon balm, on boar epididymal frozen–thawed spermatozoa quality. Testes from mature boars were collected at local slaughterhouse, and sperm samples from epididymis were recovered by flushing. Spermatozoa were cryopreserved in lactose–egg yolk buffer supplemented with various concentrations of lemon balm and mate tea (0, 2.5, 5 and 10 g l−1) using the straw‐freezing procedure. Motion parameters, acrosome and plasma membrane integrity, lipoperoxidation levels and DNA oxidative damage (8‐hydroxy‐2′‐deoxyguanosine base lesion) were evaluated. There were no differences among experimental groups with regard to motility characteristics, viability, acrosome and plasma membrane integrity; however, the highest concentration of lemon balm produced significant (P < 0.05) improvement in curvilinear trajectory, straightness and amplitude of lateral head displacement after thawing. The supplementation of freezing extender with mate tea and lemon balm reduced sperm lipid membrane peroxidation, and only mate tea protected DNA against oxidative damage during cryopreservation at 120 min post‐thawing (P < 0.05). Mate tea experimental extender at concentration of 10 g l−1 showed the lowest percentage of sperm oxidised DNA and malondialdehyde generation; thus, mate tea is a potential candidate such as antioxidant compound on boar sperm cryopreservation medium.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherWileyes_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceAndrologia 47 (9) : 1004-1011 (November 2015)es_AR
dc.subjectIlex paraguariensises_AR
dc.subjectMatees_AR
dc.subjectElissa officinalises_AR
dc.subjectAntioxidanteses_AR
dc.subjectAntioxidantseng
dc.subjectADNes_AR
dc.subjectDNAeng
dc.subjectEspermatozooes_AR
dc.subjectSpermatozoaeng
dc.subjectCriopreservaciónes_AR
dc.subjectCryopreservationeng
dc.subjectVerracoes_AR
dc.subjectBoarseng
dc.subjectCerdoes_AR
dc.subjectSwineeng
dc.subject.otherMelissa officinalises_AR
dc.subject.otherEspermaes_AR
dc.titleAntioxidant effect of lemon balm (Melissa officinalis) and mate tea (Ilex paraguensys) on quality, lipid peroxidation and DNA oxidation of cryopreserved boar epididymal spermatozoaes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEEA Balcarcees_AR
dc.description.filFil: Luño, Victoria. Universidad de Zaragoza. Facultad de Veterinaria. Departamento de Patología Animal; Españaes_AR
dc.description.filFil: Gil, María Lydia. Universidad de Zaragoza. Facultad de Veterinaria. Departamento de Patología Animal; Españaes_AR
dc.description.filFil: Olaciregui, Maite. Universidad de Zaragoza. Facultad de Veterinaria. Departamento de Patología Animal; Españaes_AR
dc.description.filFil: Jerez, Rodrigo A. Universidad de Zaragoza. Facultad de Veterinaria. Departamento de Patología Animal; Españaes_AR
dc.description.filFil: de Blas, Ignacio. Universidad de Zaragoza. Facultad de Veterinaria. Departamento de Patología Animal; Españaes_AR
dc.description.filFil: Hozbor, Federico Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentinaes_AR
dc.subtypecientifico


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