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Abstract
Previous studies in the wetland macrophyte Bidens laevis L have demonstrated that the insecticide endosulfan induces a high frequency of somatic chromosome aberrations in anaphase–telophase (CAAT) but no DNA changes as determined by the single cell gel electrophoresis (Comet) assay. Thus, cytogenetic biomarkers appear to be more sensitive to the toxic effects of the insecticide than the DNA molecule in the studied species. For this reason, the goals of [ver mas...]
dc.contributor.authorPérez, Débora Jesabel
dc.contributor.authorLukaszewicz, Germán
dc.contributor.authorMenone, Mirta Lujan
dc.contributor.authorAmé, María Valeria
dc.contributor.authorCamadro, Elsa Lucila
dc.date.accessioned2019-03-25T16:53:55Z
dc.date.available2019-03-25T16:53:55Z
dc.date.issued2014-09
dc.identifier.issn1520-4081
dc.identifier.issn1522-7278
dc.identifier.otherhttps://doi.org/10.1002/tox.21836
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/abs/10.1002/tox.21836
dc.identifier.urihttp://hdl.handle.net/20.500.12123/4729
dc.description.abstractPrevious studies in the wetland macrophyte Bidens laevis L have demonstrated that the insecticide endosulfan induces a high frequency of somatic chromosome aberrations in anaphase–telophase (CAAT) but no DNA changes as determined by the single cell gel electrophoresis (Comet) assay. Thus, cytogenetic biomarkers appear to be more sensitive to the toxic effects of the insecticide than the DNA molecule in the studied species. For this reason, the goals of this study were to use cytogenetic biomarkers—CAAT and abnormal metaphase—and defense biomarkers such as the activity of the antioxidant enzymes—guaiacol peroxidases (POD), glutathione reductase, and microsomal and cytosolic (m‐ and c‐) glutathione‐S‐transferase (GST)—to evaluate in B. laevis effects caused by a commercial formulation of endosulfan. The frequency of CAAT was increased at 5, 10, 50, and 100 μg/L endosulfan with respect to the negative controls by 3.1, 2.5, 2.5, and 3.2‐fold, respectively while the frequency of abnormal metaphases was also increased at the same concentrations by 3.5, 2.8, 3.2, and 11.3‐fold, respectively. In addition to these aneugenic effects, other abnormalities such as C‐mitosis and chromosome clumping were observed at 10 μg/L endosulfan. On the other hand, POD induction at 0.02, 0.5, 5, and 10 μg/L and m‐GST inhibition at 0.5, 10, and 50 μg/L in plants exposed during 24 h to endosulfan were observed but all of these responses were highly variable. In conclusion, only cytogenetic biomarkers like CAAT in B. laevis can serve potentially as early warning systems to detect environmentally relevant concentrations of endosulfan in aquatic ecosystems.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherWileyes_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceEnvironmental Toxicology 29 (9) : 1063-1071 (September 2014)es_AR
dc.subjectBidenses_AR
dc.subjectGenotoxicidades_AR
dc.subjectGenotoxicityeng
dc.subjectEndosulfanes_AR
dc.subjectMarcadores Genéticoses_AR
dc.subjectGenetic Markerseng
dc.subjectAcaricidases_AR
dc.subjectAcaricideseng
dc.subjectAberración Cromosómicaes_AR
dc.subjectChromosome Aberrationseng
dc.subjectPeroxídasases_AR
dc.subjectPeroxidaseseng
dc.subject.otherBidens laevises_AR
dc.subject.otherMarcadores Moleculareses_AR
dc.titleGenetic and biochemical biomarkers in the macrophyte Bidens laevis L. Exposed to a commercial formulation of endosulfanes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEEA Balcarcees_AR
dc.description.filFil: Pérez, Débora Jesabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras. Laboratorio de Ecotoxicología; Argentinaes_AR
dc.description.filFil: Lukaszewicz, Germán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras. Laboratorio de Ecotoxicología; Argentinaes_AR
dc.description.filFil: Menone, Mirta Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras. Laboratorio de Ecotoxicología; Argentinaes_AR
dc.description.filFil: Amé, María Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentinaes_AR
dc.description.filFil: Camadro, Elsa Lucila. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.subtypecientifico


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