Show simple item record

resumen

Abstract
The tomato [Solanum lycopersicum (Sl)] phosphatidylinositol‐phospholipase C (PI‐PLC) gene family is composed of six members, named SlPLC1 to SlPLC6, differentially regulated on pathogen attack. We have previously shown that the fungal elicitor xylanase induces a raise of SlPLC2 and SlPLC5 transcripts and that SlPLC2, but not SlPLC5, is required for xylanase‐induced expression of defense‐related genes. In this work we studied the role of SlPLC2 in the [ver mas...]
dc.contributor.authorGonorazky, Ana Gabriela
dc.contributor.authorGuzzo, María Carla
dc.contributor.authorAbd El Haliem, Ahmed M.
dc.contributor.authorJoosten, Matthieu H. A. J.
dc.contributor.authorLaxalt, Ana Maria
dc.date.accessioned2019-03-25T13:39:46Z
dc.date.available2019-03-25T13:39:46Z
dc.date.issued2016-12
dc.identifier.issn1364-3703 (Online)
dc.identifier.otherhttps://doi.org/10.1111/mpp.12365
dc.identifier.urihttp://hdl.handle.net/20.500.12123/4719
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/full/10.1111/mpp.12365
dc.description.abstractThe tomato [Solanum lycopersicum (Sl)] phosphatidylinositol‐phospholipase C (PI‐PLC) gene family is composed of six members, named SlPLC1 to SlPLC6, differentially regulated on pathogen attack. We have previously shown that the fungal elicitor xylanase induces a raise of SlPLC2 and SlPLC5 transcripts and that SlPLC2, but not SlPLC5, is required for xylanase‐induced expression of defense‐related genes. In this work we studied the role of SlPLC2 in the interaction between tomato and the necrotrophic fungus Botrytis cinerea. Inoculation of tomato leaves with B. cinerea increases SlPLC2 transcript levels. We knocked‐down the expression of SlPLC2 by virus‐induced gene silencing and plant defense responses were analyzed upon B. cinerea inoculation. SlPLC2 silenced plants developed smaller necrotic lesions concomitantly with less proliferation of the fungus. Silencing of SlPLC2 resulted as well in a reduced production of reactive oxygen species. Upon B. cinerea inoculation, transcript levels of the salicylic acid (SA)‐defense pathway marker gene SlPR1a were diminished in SlPLC2 silenced plants compared to non‐silenced infected plants, while transcripts of the jasmonic acid (JA)‐defense gene markers Proteinase Inhibitor I and II (SlPI‐I and SlPI‐II) were increased. This implies that SlPLC2 participates in plant susceptibility to B. cinerea.es_AR
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherWileyes_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceMolecular plant pathology 17 (9) : 1354–1363. (December 2016)es_AR
dc.subjectTomatees_AR
dc.subjectTomatoeses_AR
dc.subjectSolanum Lycopersicumes_AR
dc.subjectBotrytis Cinereaes_AR
dc.subjectFosfatidilinositoleses_AR
dc.subjectPhosphatidylinositolses_AR
dc.subjectCell Deathes_AR
dc.subjectMuerte Celulares_AR
dc.subjectJasmonic Acides_AR
dc.subjectPhospholipidses_AR
dc.subjectFosfolípidoses_AR
dc.subjectReactive Oxygen Specieses_AR
dc.subjectEspecies de Oxígeno Reactivoes_AR
dc.subjectSalicylic Acidses_AR
dc.subjectÁcido Salicílicoes_AR
dc.subjectÁcido Jasmónico
dc.subject.otherNecrotrophic Funguses_AR
dc.subject.otherPhospholipid Signallinges_AR
dc.titleSilencing of the tomato phosphatidylinositol‐phospholipase C2 (SlPLC2) reduces plant susceptibility to Botrytis cinereaes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenInstituto de Fisiología y Recursos Genéticos Vegetaleses_AR
dc.description.filFil: Gonorazky, Ana Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentinaes_AR
dc.description.filFil: Guzzo, Maria Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentinaes_AR
dc.description.filFil: Abd El Haliem, Ahmed M. Wageningen University. Laboratory of Phytopathology; Holandaes_AR
dc.description.filFil: Joosten, Matthieu H. A. J. Wageningen University. Laboratory of Phytopathology; Holandaes_AR
dc.description.filFil: Laxalt, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentinaes_AR
dc.subtypecientifico


Files in this item

Thumbnail

This item appears in the following Collection(s)

common

Show simple item record