Mostrar el registro sencillo del ítem
resumen
Resumen
Potato leaves infected with Phytophthora infestans produced a serine protease inhibitor (PLPKI) with specificity for microbial proteases. Sequencing of the first twenty residues at the NH2‐terminus of the mature PLPKI polypeptide demonstrated that PLPKI is a novel member of the potato protease inhibitor I family. PLPKI inhibited the activity of extracellular proteases produced by two pathogens of potato, P. infestans and Rhizoctonia solani, but was
[ver mas...]
dc.contributor.author | Feldman, Mariana Laura | |
dc.contributor.author | Andreu, Adriana Balbina | |
dc.contributor.author | Korgan, Samanta | |
dc.contributor.author | Lobato, Maria Candela | |
dc.contributor.author | Huarte, Marcelo Atilio | |
dc.contributor.author | Walling, Linda Lee | |
dc.contributor.author | Daleo, Gustavo Raul | |
dc.date.accessioned | 2019-03-25T13:01:05Z | |
dc.date.available | 2019-03-25T13:01:05Z | |
dc.date.issued | 2014-04 | |
dc.identifier.issn | 0179-9541 | |
dc.identifier.issn | 1439-0523 | |
dc.identifier.other | https://doi.org/10.1111/pbr.12129 | |
dc.identifier.uri | https://onlinelibrary.wiley.com/doi/10.1111/pbr.12129 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/4718 | |
dc.description.abstract | Potato leaves infected with Phytophthora infestans produced a serine protease inhibitor (PLPKI) with specificity for microbial proteases. Sequencing of the first twenty residues at the NH2‐terminus of the mature PLPKI polypeptide demonstrated that PLPKI is a novel member of the potato protease inhibitor I family. PLPKI inhibited the activity of extracellular proteases produced by two pathogens of potato, P. infestans and Rhizoctonia solani, but was inactive against proteases secreted into the culture media by the binucleate Rhizoctonia N2, a non‐pathogenic fungus for potato. Western blot analysis showed a positive correlation between the levels of PLPKI and the degree of horizontal resistance, showing its highest accumulation in clone OKA 5632.11, which has been described as highly resistant. This correlation, together with the ability of PLPKI to completely abolish the secreted serine protease activity of P. infestans, suggests that PLPKI may have an active role in protecting potato plants from this pathogenic oomycete and that it could be used as a suitable biochemical marker to help breeders in the selection of cultivars with high degree of horizontal resistance. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.publisher | Wiley | es_AR |
dc.rights | info:eu-repo/semantics/restrictedAccess | es_AR |
dc.source | Plant Breeding 133 (2) : 275-280 (April 2014) | es_AR |
dc.subject | Phytophthora infestans | es_AR |
dc.subject | Papa | es_AR |
dc.subject | Potatoes | eng |
dc.subject | Enfermedades de las Plantas | es_AR |
dc.subject | Plant Diseases | eng |
dc.subject | Resistencia a la Enfermedad | es_AR |
dc.subject | Disease Resistance | eng |
dc.subject | Serina | es_AR |
dc.subject | Serine | eng |
dc.subject | Inhibidores de Proteinasas | es_AR |
dc.subject | Proteinase Inhibitors | eng |
dc.title | PLPKI: A novel serine protease inhibitor as a potential biochemical marker involved in horizontal resistance to Phytophthora infestans | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.description.origen | EEA Balcarce | es_AR |
dc.description.fil | Fil: Feldman, Mariana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina | es_AR |
dc.description.fil | Fil: Andreu, Adriana Balbina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina | es_AR |
dc.description.fil | Fil: Korgan, Samanta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina | es_AR |
dc.description.fil | Fil: Lobato, Maria Candela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina | es_AR |
dc.description.fil | Fil: Huarte, Marcelo Atilio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina | es_AR |
dc.description.fil | Fil: Walling, Linda Lee. University of California. Department of Botany and Plant Sciences; Estados Unidos | es_AR |
dc.description.fil | Fil: Daleo, Gustavo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina | es_AR |
dc.subtype | cientifico |
Ficheros en el ítem
Este ítem aparece en la(s) siguiente(s) colección(ones)
common
-
Artículos científicos [619]