Mostrar el registro sencillo del ítem
resumen
Resumen
This study was conducted to evaluate the effect of seminal collection method (artificial vagina or electroejaculation) on the protein composition of seminal plasma and sperm quality parameters in Corriedale rams. To address this question, we assessed the effect of seminal collection method on motility, plasma membrane integrity and functionality, mitochondrial functionality and the decondensation state of nuclear chromatin in sperm cells. Volume, pH,
[ver mas...]
dc.contributor.author | Ledesma, Alba | |
dc.contributor.author | Manes, Jorgelina | |
dc.contributor.author | Cesari, Andreina | |
dc.contributor.author | Alberio, Ricardo | |
dc.contributor.author | Hozbor, Federico Andres | |
dc.date.accessioned | 2019-03-22T14:15:26Z | |
dc.date.available | 2019-03-22T14:15:26Z | |
dc.date.issued | 2014-04 | |
dc.identifier.issn | 0936-6768 | |
dc.identifier.issn | 1439-0531 | |
dc.identifier.other | https://doi.org/10.1111/rda.12279 | |
dc.identifier.uri | https://onlinelibrary.wiley.com/doi/abs/10.1111/rda.12279 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/4707 | |
dc.description.abstract | This study was conducted to evaluate the effect of seminal collection method (artificial vagina or electroejaculation) on the protein composition of seminal plasma and sperm quality parameters in Corriedale rams. To address this question, we assessed the effect of seminal collection method on motility, plasma membrane integrity and functionality, mitochondrial functionality and the decondensation state of nuclear chromatin in sperm cells. Volume, pH, osmolarity, protein concentration, total protein content and protein profile using sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS‐PAGE) and 2‐D polyacrylamide electrophoresis of seminal plasma collected with artificial vagina and electroejaculation were also analysed. The main findings from this study were that ejaculates obtained with electroejaculation had (i) a higher number of spermatozoa with intact plasma membrane and functional mitochondria and (ii) a higher proportion of seminal plasma, total protein content and relative abundance of low molecular weight proteins than ejaculates obtained with artificial vagina. Five of these proteins were identified by mass spectrometry: binder of sperm 5 precursor; RSVP14; RSVP22; epididymal secretory protein E1 and clusterin. One protein spot with molecular weight of approximately 31 kDa and isoelectric point of 4.8 was only found in the seminal plasma from electroejaculation. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.publisher | Wiley | es_AR |
dc.rights | info:eu-repo/semantics/restrictedAccess | es_AR |
dc.source | Reproduction in Domestic Animals 49 (2) : 324-332 (April 2014) | es_AR |
dc.subject | Morueco | es_AR |
dc.subject | Rams | eng |
dc.subject | Ovinos | es_AR |
dc.subject | Sheep | eng |
dc.subject | Razas (animales) | es_AR |
dc.subject | Breeds (animals) | eng |
dc.subject | Espermatozoo | es_AR |
dc.subject | Spermatozoa | eng |
dc.subject | Semen | es_AR |
dc.subject | Electroeyaculación | es_AR |
dc.subject | Electroejaculation | eng |
dc.subject.other | Carnero padre | es_AR |
dc.subject.other | Raza Corriedale | es_AR |
dc.subject.other | Esperma | es_AR |
dc.subject.other | Plasma seminal | es_AR |
dc.title | Electroejaculation Increases Low Molecular Weight Proteins in Seminal Plasma Modifying Sperm Quality in Corriedale Rams | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.description.origen | EEA Balcarce | es_AR |
dc.description.fil | Fil: Ledesma, Alba. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina | es_AR |
dc.description.fil | Fil: Manes, Jorgelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina. | es_AR |
dc.description.fil | Fil: Cesari, Andreina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina | es_AR |
dc.description.fil | Fil: Alberio, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina | es_AR |
dc.description.fil | Fil: Hozbor, Federico Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina | es_AR |
dc.subtype | cientifico |
Ficheros en el ítem
Este ítem aparece en la(s) siguiente(s) colección(ones)
common
-
Artículos científicos [619]