Mostrar el registro sencillo del ítem
resumen
Resumen
A Plum pox virus (PPV) isolate detected in a Japanese plum orchard in Pocito
(San Juan, Argentina) was transmitted mechanically to Prunus tomentosa
and Nicotiana benthamiana. DAS-ELISA and DASI-ELISA indicated the
virus presence and serological relationship with D-strain isolates; ICRT-
PCR amplified a 1.2-kb fragment of the virus genome encoding the
CP-3′ nc region. The analysis of the sequence showed the presence of the
DAG motif at the 5′ end of
[ver mas...]
dc.contributor.author | Dal Zotto, Angelica | |
dc.contributor.author | Balzarini, Mónica Graciela | |
dc.contributor.author | Raigon, Juan Manuel | |
dc.contributor.author | Rossini, Mirta Noemi | |
dc.contributor.author | Ducasse, Daniel Adrian | |
dc.date.accessioned | 2019-03-19T12:03:54Z | |
dc.date.available | 2019-03-19T12:03:54Z | |
dc.date.issued | 2014-01 | |
dc.identifier.issn | 1439-0434 (Online) | |
dc.identifier.other | https://doi.org/10.1111/jph.12160 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/4648 | |
dc.description.abstract | A Plum pox virus (PPV) isolate detected in a Japanese plum orchard in Pocito (San Juan, Argentina) was transmitted mechanically to Prunus tomentosa and Nicotiana benthamiana. DAS-ELISA and DASI-ELISA indicated the virus presence and serological relationship with D-strain isolates; ICRT- PCR amplified a 1.2-kb fragment of the virus genome encoding the CP-3′ nc region. The analysis of the sequence showed the presence of the DAG motif at the 5′ end of the capsid protein and the Rsa I and Alu I sites at the 3′ end. The phylogenetic relationships and multiple alignment with PPV isolates from NCBI database indicated greatest (+98%) homology with the D strain and close identity with MNAT1 (\AF360579) USA peach isolate. The sequence analysed showed two amino acid mutations towards the 5′ N-terminus of CP (the most variable region) with respect to a consensus of PPV D-strain isolates. This is the first molecular characterization of 3′terminal genome region of PPV isolate to confirm D strain in a Japanese plum from Argentina. | eng |
dc.format | application/pdf | eng |
dc.language.iso | eng | |
dc.publisher | Wiley | eng |
dc.rights | info:eu-repo/semantics/restrictedAccess | eng |
dc.source | Journal of phytopathology 162 (1) : 55-60. (January 2014) | eng |
dc.subject | Prunus Salicina | es_AR |
dc.subject | Plum Pox Virus | eng |
dc.subject | Virus Sharka del Ciruelo | es_AR |
dc.subject | Variedades | es_AR |
dc.subject | Varieties | eng |
dc.subject.other | Ciruelo Japonés | es_AR |
dc.subject.other | Argentina | es_AR |
dc.subject.other | Red Beauty (Variedad) | es_AR |
dc.subject.other | Phylogenetic Relationships | eng |
dc.subject.other | Relación Filogenética | es_AR |
dc.title | Plum Pox virus in japanese plum from Argentina: serological detection and molecular characterization of an isolate from cv. Red Beauty | eng |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | eng |
dc.type | info:eu-repo/semantics/publishedVersion | eng |
dc.description.origen | Instituto de Patología Vegetal | es_AR |
dc.description.fil | Fil: Dal Zotto, Angelica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina | es_AR |
dc.description.fil | Fil: Balzarini, Mónica Graciela. Universidad Nacional de Cordoba. Facultad de Ciencias Agropecuarias; Argentina | es_AR |
dc.description.fil | Fil: Raigon, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan. Agencia de Extensión Rural San Martín; Argentina | es_AR |
dc.description.fil | Fil: Rossini, Mirta Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Alto Valle; Argentina | es_AR |
dc.description.fil | Fil: Ducasse, Daniel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina | es_AR |
dc.subtype | cientifico |
Ficheros en el ítem
Este ítem aparece en la(s) siguiente(s) colección(ones)
common
-
Artículos científicos [237]