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Abstract
Background: Saliva of mosquitoes contains anti-platelet, anti-clotting, vasodilatory, anti-complement and antiinflammatory substances that help the blood feeding process. The salivary polypeptides are at a fast pace of evolution possibly due to their relative lack of structural constraint and possibly also by positive selection on their genes leading to evasion of host immune pressure. Results: In this study, we used deep mRNA sequence to uncover for [ver mas...]
dc.contributor.authorScarpassa, Vera Margarete
dc.contributor.authorDebat, Humberto Julio
dc.contributor.authorAlencar, Ronildo Baiatone
dc.contributor.authorFerreira Saraiva, José
dc.contributor.authorCalvo, Eric
dc.contributor.authorArcà, Bruno
dc.contributor.authorRibeiro, José M. C.
dc.date.accessioned2019-03-18T18:47:22Z
dc.date.available2019-03-18T18:47:22Z
dc.date.issued2019
dc.identifier.issn1471-2164
dc.identifier.otherhttps://doi.org/10.1186/s12864-019-5545-0
dc.identifier.urihttp://hdl.handle.net/20.500.12123/4644
dc.identifier.urihttps://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-019-5545-0
dc.description.abstractBackground: Saliva of mosquitoes contains anti-platelet, anti-clotting, vasodilatory, anti-complement and antiinflammatory substances that help the blood feeding process. The salivary polypeptides are at a fast pace of evolution possibly due to their relative lack of structural constraint and possibly also by positive selection on their genes leading to evasion of host immune pressure. Results: In this study, we used deep mRNA sequence to uncover for the first time the sialomes of four Amazonian anophelines species (Anopheles braziliensis, A. marajorara, A. nuneztovari and A. triannulatus) and extend the knowledge of the A. darlingi sialome. Two libraries were generated from A. darlingi mosquitoes, sampled from two localities separated ~ 1100 km apart. A total of 60,016 sequences were submitted to GenBank, which will help discovery of novel pharmacologically active polypeptides and the design of specific immunological markers of mosquito exposure. Additionally, in these analyses we identified and characterized novel phasmaviruses and anpheviruses associated to the sialomes of A. triannulatus, A. marajorara and A. darlingi species. Conclusions: Besides their pharmacological properties, which may be exploited for the development of new drugs (e.g. anti-thrombotics), salivary proteins of blood feeding arthropods may be turned into tools to prevent and/or better control vector borne diseases; for example, through the development of vaccines or biomarkers to evaluate human exposure to vector bites. The sialotranscriptome study reported here provided novel data on four New World anopheline species and allowed to extend our knowledge on the salivary repertoire of A. darlingi. Additionally, we discovered novel viruses following analysis of the transcriptomes, a procedure that should become standard within future RNAseq studies.es_AR
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherBioMed Centrales_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourceBMC genomics 20:166. (2019)es_AR
dc.subjectCulicidaees_AR
dc.subjectMalariaes_AR
dc.subjectViroseses_AR
dc.subjectVirosises_AR
dc.subjectSalivary Glandses_AR
dc.subjectGlándulas Salivaleses_AR
dc.subjectVectoreses_AR
dc.subjectVectorses_AR
dc.subjectAnopheleses_AR
dc.subject.otherTranscriptomees_AR
dc.titleAn insight into the sialotranscriptome and virome of Amazonian anophelineses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenInstituto de Patología Vegetales_AR
dc.description.filFil: Scarpassa, Vera Margarete .Instituto Nacional de Pesquisas da Amazônia. Coordenação de Biodiversidade. Laboratório de Genética de Populações e Evolução de Mosquitos Vetores de Malária e Dengue; Brasiles_AR
dc.description.filFil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentinaes_AR
dc.description.filFil: Alencar, Ronildo Baiatone. Instituto Nacional de Pesquisas da Amazônia. Coordenação de Biodiversidade. Laboratório de Genética de Populações e Evolução de Mosquitos Vetores de Malária e Dengue; Brasiles_AR
dc.description.filFil: Ferreira Saraiva, José. Instituto Nacional de Pesquisas da Amazônia. Coordenação de Biodiversidade. Laboratório de Genética de Populações e Evolução de Mosquitos Vetores de Malária e Dengue; Brasiles_AR
dc.description.filFil: Calvo, Eric. National Institute of Allergy and Infectious Diseases. Laboratory of Malaria and Vector Research; Estados Unidoses_AR
dc.description.filFil: Arcà, Bruno. Sapienza University of Rome. Division of Parasitology. Department of Public Health and Infectious Diseaseses_AR
dc.description.filFil: Ribeiro, José M. C. Instituto Nacional de Pesquisas da Amazônia. Coordenação de Biodiversidade. Laboratório de Genética de Populações e Evolução de Mosquitos Vetores de Malária e Dengue; Brasiles_AR
dc.subtypecientifico


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