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Abstract
The increase in human babesiosis is of major concern to health authorities. In the USA, most of these cases are due to infections with Babesia microti, whereas in Europe B. divergens is the major cause of clinical disease in humans. Here we review the immunological and biological literature of glycosylphosphatidylinositol (GPI)-anchored merozoite proteins of human Babesia parasites with emphasis on their role in immunity, and provide some new [ver mas...]
dc.contributor.authorWieser, Sarah Nathaly
dc.contributor.authorSchnittger, Leonhard
dc.contributor.authorFlorin-Christensen, Monica
dc.contributor.authorDelbecq, Stephane
dc.contributor.authorSchetters, Theo
dc.date.accessioned2019-02-19T17:46:38Z
dc.date.available2019-02-19T17:46:38Z
dc.date.issued2019-01
dc.identifier.issn0020-7519
dc.identifier.otherhttps://doi.org/10.1016/j.ijpara.2018.12.002
dc.identifier.urihttp://hdl.handle.net/20.500.12123/4469
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0020751919300037?via%3Dihub
dc.descriptionIn Press, Corrected Proof
dc.description.abstractThe increase in human babesiosis is of major concern to health authorities. In the USA, most of these cases are due to infections with Babesia microti, whereas in Europe B. divergens is the major cause of clinical disease in humans. Here we review the immunological and biological literature of glycosylphosphatidylinositol (GPI)-anchored merozoite proteins of human Babesia parasites with emphasis on their role in immunity, and provide some new bioinformatical information on B. microti GPI-Anchored Proteins (GPI-AP). Cattle can be vaccinated with soluble parasite antigens (SPA) of Babesia divergens that are released by the parasite during proliferation. The major component in SPA preparations appeared to be a 37 kDa merozoite surface protein that is anchored in the merozoite membrane by a GPI anchor. Animals could be protected by vaccination with the recombinant 37 kDa protein expressed in Escherichia coli, provided the protein had a hydrophobic terminal sequence. Based on this knowledge, a recombinant vaccine was developed against Babesia canis infection in dogs, successfully. In order to identify similar GPI-AP in B. microti, the genome was analysed. Here it is shown that B. microti encodes all proteins necessary for GPI assembly and its subsequent protein transfer. In addition, in total 21 genes encoding for GPI-AP were detected, some of which reacted particularly strongly with sera from B. microti-infected human patients. Reactivity of antibodies with GPI-anchored merozoite proteins appears to be dependent on the structural conformation of the molecule. It is suggested that the three-dimensional structure of the protein that is anchored in the membrane is different from that of the protein that has been shed from the merozoite surface. The significance of this protein’s dynamics in parasite biology and immune evasion is discussed. Finally, we discuss developments in tick and Babesia vaccine research, and the role such vaccines could play in the control of human babesiosis.eng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.publisherElseviereng
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.sourceInternational journal for parasitology (24 January 2019)eng
dc.subjectBabesiosises_AR
dc.subjectVaccinationeng
dc.subjectVacunaciónes_AR
dc.subjectSporozoaeng
dc.subjectBabesia Divergenses_AR
dc.subjectBabesia Microties_AR
dc.subjectSynthetic Vaccineseng
dc.subjectVacuna Sintéticaes_AR
dc.subject.otherApicomplexaes_AR
dc.subject.otherGPI Anchorseng
dc.subject.otherMerozoite Surface Proteineng
dc.subject.otherGlycosylphosphatidylinositoleng
dc.titleVaccination against babesiosis using recombinant GPI-anchored proteinseng
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenInstituto de Patobiologíaes_AR
dc.description.filFil: Wieser, Sarah Nathaly. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Delbecq, Stephane. Université de Montpellier.· Vaccination Antiparasitaire; Franciaes_AR
dc.description.filFil: Schetters, Theo. Université de Montpellier.· Vaccination Antiparasitaire; Francia. University of Pretoria. Veterinary Faculty. Department of Veterinary Tropical Diseases; Sudáfricaes_AR
dc.subtypecientifico


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