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The protozoon Sarcocystis aucheniae is the causative agent of South American camelid (SAC) sarcocystosis. Infections are characterized by the presence of cysts in muscles which are in size and appearance similar to rice grains. As consumption of insufficiently cooked infected meat produces gastroenteritis, cyst-containing SAC meat is confiscated by sanitary authorities or depreciated with serious economic consequences for SAC breeders. In this work, a [ver mas...]
dc.contributor.authorDecker Franco, Cecilia
dc.contributor.authorRomero, Sandra Raquel
dc.contributor.authorFerrari, Alejandro
dc.contributor.authorSchnittger, Leonhard
dc.contributor.authorFlorin-Christensen, Mónica
dc.date.accessioned2019-02-13T14:46:53Z
dc.date.available2019-02-13T14:46:53Z
dc.date.issued2018
dc.identifier.issn2405-8440
dc.identifier.otherhttps://doi.org/10.1016/j.heliyon.2018.e00928
dc.identifier.urihttp://hdl.handle.net/20.500.12123/4436
dc.identifier.urihttps://www.heliyon.com/article/e00928
dc.description.abstractThe protozoon Sarcocystis aucheniae is the causative agent of South American camelid (SAC) sarcocystosis. Infections are characterized by the presence of cysts in muscles which are in size and appearance similar to rice grains. As consumption of insufficiently cooked infected meat produces gastroenteritis, cyst-containing SAC meat is confiscated by sanitary authorities or depreciated with serious economic consequences for SAC breeders. In this work, a duplex semi-nested PCR was designed to simultaneously detect parasite and llama DNA in host blood samples. Species-specific regions of S. aucheniae 18S rRNA gene and Lama glama 16S mitochondrial gene were amplified, yielding bands of 583 and 257 bp, respectively, and separated by gel electrophoresis. The method proved to be highly sensitive, with a detection limit lower than one parasite per milliliter blood, and the inclusion of primers to detect llama-specific DNA resulted useful as a methodological control. Blood samples collected from llamas of Argentina and Bolivia (n = 225) were analyzed using this method, and 18.7 % resulted positive for S. aucheniae. No correlation was found between PCR results and llama age, sex or the finding of macroscopic cysts in meat after slaughter. Lack of molecular detection in the blood of some llamas harboring macrocysts suggests that parasite circulation in the bloodstream after encystment is under the detection threshold of the test or even absent, while PCR positive results in cyst-infested animals suggests that prior exposure to the parasite does not impede subsequent infections. The described method can be useful to detect active foci of infection, to assess the effectiveness of parasiticide treatments, and for the surveillance and tracing of definitive hosts.eng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.publisherElsevieres_AR
dc.rightsinfo:eu-repo/semantics/openAccesseng
dc.sourceHeliyon 4 : e00928. (2018)eng
dc.subjectLlamaes_AR
dc.subjectSangrees_AR
dc.subjectBloodeng
dc.subjectPCRes_AR
dc.subjectInfestaciónes_AR
dc.subjectInfestationeng
dc.subjectInfecciones por Protozooses_AR
dc.subjectProtozoal Infectionseng
dc.subject.otherSarcocystis Aucheniaees_AR
dc.titleDetection of Sarcocystis aucheniae in blood of llama using a duplex semi-nested PCR assay and its association with cyst infestationeng
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenInstituto de Patobiologíaes_AR
dc.description.filFil: Decker Franco, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Romero, Sandra Raquel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación y Desarrollo Tecnológico para la Agricultura Familiar Región NOA; Argentinaes_AR
dc.description.filFil: Ferrari, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentinaes_AR
dc.description.filFil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Florin-Christensen, Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.subtypecientifico


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