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Effect of Bovine Viral Diarrhea Virus on the ovarian functionality and in vitro reproductive performance of persistently infected heifers

Abstract
The aim of this study was to study the effect of Bovine Viral Diarrhea Virus on the reproductive female tract by means of analyzing the ovarian follicular population of persistently infected (PI) heifers, and evaluating the performance of oocytes procured form those heifers in in vitro fertilization procedures. Seven BVDV PI Aberdeen Angus and British crossbred heifers ranging from 18 to 36 months of age were spayed and their ovaries used for viral [ver mas...]
The aim of this study was to study the effect of Bovine Viral Diarrhea Virus on the reproductive female tract by means of analyzing the ovarian follicular population of persistently infected (PI) heifers, and evaluating the performance of oocytes procured form those heifers in in vitro fertilization procedures. Seven BVDV PI Aberdeen Angus and British crossbred heifers ranging from 18 to 36 months of age were spayed and their ovaries used for viral isolation, microscopic examination, and in vitro fertilization procedures. Bovine Viral Diarrhea Virus was detected from the follicular fluid and sera of all PI heifers. Microscopic examination of the ovaries from PI heifers showed a significant drop in the number of follicles cortical regions, compared with controls. A comparative analysis of the stages of follicular development showed a significant decrease in the number of primordial and tertiary follicles in the cortical regions of ovaries from PI heifers. Viral antigen was detected by immunohistochemistry, and was widely distributed throughout the ovarian tissues. There were differences in the rate of cleavage and embryo development between oocytes obtained from the ovaries of control animals and PI heifers. Furthermore, two developed embryos obtained from oocytes from one of the PI heifers were positive to BVDV, as well as two media from in vitro fertilization (IVF) procedures. The results of this study demonstrate that BVDV PI heifers exhibit alterations in follicular population through of the early interaction between the virus and germ cell line affecting directly the mechanisms involved in the ontogenesis of the ovary. [Cerrar]
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Author
González Altamiranda, Erika Analía;   Kaiser, German Gustavo;   Mucci, Nicolas Crescencio;   Verna, Andrea Elizabeth;   Campero, Carlos Manuel;   Odeon, Anselmo Carlos;  
Fuente
Veterinary Microbiology 165 (3–4) : 326-332 (August 2013)
Date
2013-08-30
Editorial
Elsevier
ISSN
0378-1135
URI
https://www.sciencedirect.com/science/article/pii/S0378113513002095
http://hdl.handle.net/20.500.12123/4329
DOI
https://doi.org/10.1016/j.vetmic.2013.04.007
Formato
pdf
Tipo de documento
artículo
Palabras Claves
Novilla; Heifers; Enfermedades de los Animales; Animal Diseases; Pestivirus de la Diarrea Bovina; Bovine Diarrhoea Pestivirus; Diarrea Viral Bovina; Bovine Viral Diarrhoea; Folículos Ováricos; Ovarian Follicles;
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Excepto donde se diga explicitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
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