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Abstract
Goats are susceptible to brucellosis and the detection of Brucella-infected animals is carried out by serological tests. In other ruminant species, polymorphisms in microsatellites (Ms) of 3′ untranslated region (3′UTR) of the solute carrier family 11 member A1 (SLC11A1) gene were associated with resistance to Brucella abortus infection. Goats present two polymorphic Ms at the 3′UTR end of SLC11A1 gene, called regions A and B. Here, we evaluated if [ver mas...]
dc.contributor.authorIacoboni, Paola Anabella
dc.contributor.authorHasenauer, Flavia Carolina
dc.contributor.authorCaffaro, María Eugenia
dc.contributor.authorGaido, Analia Beatriz
dc.contributor.authorRossetto, Cristina
dc.contributor.authorNeumann, Roberto Daniel
dc.contributor.authorSalatin, Antonio Omar
dc.contributor.authorBertoni, Emiliano Agustín
dc.contributor.authorPoli, Mario Andres
dc.contributor.authorRossetti, Carlos Alberto
dc.date.accessioned2019-01-23T13:01:40Z
dc.date.available2019-01-23T13:01:40Z
dc.date.issued2014-08-15
dc.identifier.issn0165-2427
dc.identifier.otherhttps://doi.org/10.1016/j.vetimm.2014.05.007
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0165242714001202
dc.identifier.urihttp://hdl.handle.net/20.500.12123/4315
dc.description.abstractGoats are susceptible to brucellosis and the detection of Brucella-infected animals is carried out by serological tests. In other ruminant species, polymorphisms in microsatellites (Ms) of 3′ untranslated region (3′UTR) of the solute carrier family 11 member A1 (SLC11A1) gene were associated with resistance to Brucella abortus infection. Goats present two polymorphic Ms at the 3′UTR end of SLC11A1 gene, called regions A and B. Here, we evaluated if polymorphisms in regions A and/or B are associated with Brucella infection in goats. Serum (for the detection of Brucella-specific antibodies) and hair samples (for DNA isolation and structure analysis of the SLC11A1 gene) were randomly collected from 229 adult native goats from the northwest of Argentina. Serological status was evaluated by buffer plate antigen test (BPAT) complemented by the fluorescent polarization assay (FPA), and the genotype of the 3′UTR of the SLC11A1 gene was determined by capillary electrophoresis and confirmed by sequence analysis. Polymorphisms in regions A and B of the 3′UTR SLC11A1 gene were found statistically significant associated with protection to Brucella infection. Specifically, the association study indicates statistical significance of the allele A15 and B7/B7 genotype with absence of Brucella-specific antibodies (p = 0.0003 and 0.0088, respectively). These data open a promising opportunity for limiting goat brucellosis through selective breeding of animals based on genetic markers associated with natural resistance to B. melitensis infection.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherElsevieres_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceVeterinary Immunology and Immunopathology 160 (3–4) : 230-234 (August 2014)es_AR
dc.subjectCaprinoses_AR
dc.subjectGoatseng
dc.subjectGenéticaes_AR
dc.subjectGeneticseng
dc.subjectPolimorfismoes_AR
dc.subjectPolymorphismeng
dc.subjectBrucellaes_AR
dc.subjectBrucelosises_AR
dc.subjectBrucellosiseng
dc.titlePolymorphisms at the 3′ untranslated region of SLC11A1 gene are associated with protection to Brucella infection in goatses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Patobiologíaes_AR
dc.description.filFil: Iacoboni, Paola Anabella. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentinaes_AR
dc.description.filFil: Hasenauer, Flavia Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Caffaro, Marí­a Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentinaes_AR
dc.description.filFil: Gaido, Analía Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentinaes_AR
dc.description.filFil: Rossetto, Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Yuto; Argentinaes_AR
dc.description.filFil: Neumann, Roberto Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentinaes_AR
dc.description.filFil: Salatin, Antonio Omar. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentinaes_AR
dc.description.filFil: Bertoni, Emiliano Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentinaes_AR
dc.description.filFil: Poli, Mario Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentinaes_AR
dc.description.filFil: Rossetti, Carlos Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentinaes_AR
dc.subtypecientifico


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