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Abstract
Mycobacterium bovis is the causative agent of bovine tuberculosis, a disease that affects approximately 5% of Argentine cattle. The aim of this research was to study if it is possible to infer the degree of virulence of different M. bovis genotypes based on scorified observations of tuberculosis lesions in cattle. In this study, we performed association analyses between several parameters with tuberculosis lesions: M. bovis genotype, degree of progression [ver mas...]
dc.contributor.authorGarbaccio, Sergio Gabriel
dc.contributor.authorMacias, Analía
dc.contributor.authorShimizu, Ernesto
dc.contributor.authorPaolicchi, Fernando
dc.contributor.authorPezzone, Natalia
dc.contributor.authorZapata, Laura
dc.contributor.authorAbdala, Alejandro Ariel
dc.contributor.authorTarabla, Hector Dante
dc.contributor.authorPeyru, Maite
dc.contributor.authorCaimi, Karina Cynthia
dc.contributor.authorZumarraga, Martin Jose
dc.contributor.authorCanal, Ana María
dc.contributor.authorCataldi, Angel Adrian
dc.contributor.authorMagnano, Gabriel
dc.date.accessioned2019-01-18T13:48:36Z
dc.date.available2019-01-18T13:48:36Z
dc.date.issued2014-01
dc.identifier.issn2150-5594
dc.identifier.issn2150-5608
dc.identifier.otherhttps://doi.org/10.4161/viru.27193
dc.identifier.urihttps://www.tandfonline.com/doi/full/10.4161/viru.27193
dc.identifier.urihttp://hdl.handle.net/20.500.12123/4294
dc.description.abstractMycobacterium bovis is the causative agent of bovine tuberculosis, a disease that affects approximately 5% of Argentine cattle. The aim of this research was to study if it is possible to infer the degree of virulence of different M. bovis genotypes based on scorified observations of tuberculosis lesions in cattle. In this study, we performed association analyses between several parameters with tuberculosis lesions: M. bovis genotype, degree of progression of tuberculosis, and animal age. For this purpose, the genotype was determined by spoligotyping and the degree of bovine tuberculosis gross lesion was quantified with a score based on clinical observations (number, size, and location of granulomas along with histopathologic features). This study was performed with naturally infected cattle of slaughterhouses from three provinces in Argentina. A total of 265 M. bovis isolates were obtained from 378 pathological lesion samples and 192 spoligotyping and VNTR (based on ETR sequences) typing patterns were obtained. SB0140 was the most predominant spoligotype, followed by SB0145. The spoligotype with the highest lesion score was SB0273 (median score of 27 ± 4.46), followed by SB0520 (18 ± 5.8). Furthermore, the most common spoligotype, SB0140, had a median score of 11 ± 0.74. Finally, the spoligotype with the lowest score was SB0145 (8 ± 1.0). ETR typing of SB0140, SB0145, SB0273, and SB0520 did not subdivide the lesion scores in those spoligotypes. In conclusion, SB0273 and SB0520 were the spoligotypes with the strongest association with hypervirulence and both spoligotypes were only found in Río Cuarto at the south of Córdoba province. Interestingly, there is no other report of any of these spoligotyes in Latin America.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherTaylor & Francises_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourceVirulence 5 (2) : 297-302 (2014)es_AR
dc.subjectMycobacterium Bovises_AR
dc.subjectGanado Bovinoes_AR
dc.subjectCattleeng
dc.subjectEnfermedades de los Animaleses_AR
dc.subjectAnimal Diseaseseng
dc.subjectVirulenciaes_AR
dc.subjectVirulenceeng
dc.subjectGenotiposes_AR
dc.subjectGenotypeseng
dc.titleAssociation between spoligotype-VNTR types and virulence of Mycobacterium bovis in cattlees_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Patobiologíaes_AR
dc.description.filFil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentinaes_AR
dc.description.filFil: Macias, Analía. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentinaes_AR
dc.description.filFil: Shimizu, Ernesto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Bacteriología; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentinaes_AR
dc.description.filFil: Paolicchi, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Bacteriología; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentinaes_AR
dc.description.filFil: Pezzone, Natalia. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Patología Básica; Argentinaes_AR
dc.description.filFil: Magnano, Gabriel. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentinaes_AR
dc.description.filFil: Zapata, Laura. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentinaes_AR
dc.description.filFil: Abdala, Alejandro Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentinaes_AR
dc.description.filFil: Tarabla, Hector Dante. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentinaes_AR
dc.description.filFil: Peyru, Maite. Entre Ríos (provincia). Secretaría de la Producción de Entre Ríos. Dirección General de Producción Animal; Argentinaes_AR
dc.description.filFil: Caimi, Karina Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Canal, Ana María. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Patología Básica; Argentinaes_AR
dc.description.filFil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.subtypecientifico


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