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Abstract
Leptospirosis is an important global zoonotic disease caused by pathogenic Leptospira spp. species. Swine leptospirosis has a major economic impact because pigs are sources of animal protein and by-products. The signs of swine leptospirosis are abortion, stillbirth, birth of weak or ill piglets, appearing 14–60 days after infection. The reference method for diagnosis of leptospirosis is the microscopic agglutination test (MAT), in which serum samples are [ver mas...]
dc.contributor.authorHartleben, Cláudia P.
dc.contributor.authorLeal, Fernanda M. A.
dc.contributor.authorMonte, Leonardo G.
dc.contributor.authorHartwig, Daiane D.
dc.contributor.authorSeixas, Fabiana K.
dc.contributor.authorVasconcellos, Sílvio A.
dc.contributor.authorBrihuega, Bibiana Felicitas
dc.contributor.authorDellagostin, Odir A.
dc.date.accessioned2019-01-16T13:59:11Z
dc.date.available2019-01-16T13:59:11Z
dc.date.issued2013-02
dc.identifier.issn0343-8651
dc.identifier.issn1432-0991
dc.identifier.otherhttps://doi.org/10.1007/s00284-012-0237-x
dc.identifier.urihttps://link.springer.com/article/10.1007%2Fs00284-012-0237-x
dc.identifier.urihttp://hdl.handle.net/20.500.12123/4273
dc.description.abstractLeptospirosis is an important global zoonotic disease caused by pathogenic Leptospira spp. species. Swine leptospirosis has a major economic impact because pigs are sources of animal protein and by-products. The signs of swine leptospirosis are abortion, stillbirth, birth of weak or ill piglets, appearing 14–60 days after infection. The reference method for diagnosis of leptospirosis is the microscopic agglutination test (MAT), in which serum samples are reacted with live antigen suspensions of leptospiral serovars. However, MAT is laborious and time consuming as a diagnostic procedure when dealing with a large number of samples; therefore, efforts are being made to develop novel, sensitive, and specific diagnostic tests for leptospirosis. In this study, a recombinant LipL32 based on enzyme-linked immunosorbent assay (rLipL32/ELISA) was evaluated as a screening test for the detection of pathogenic leptospiral-specific antibodies. A total of 86 swine serum samples tested by MAT were used to develop rLipL32/ELISA. Compared to positive and negative sera tested by MAT, rLipL32/ELISA showed 100 % sensitivity, 85.1 % specificity, and 91.86 % accuracy. No positive reaction for other bacterial diseases (enzootic pneumonia and brucellosis) was observed. The rLipL32/ELISA reported in this study is a specific, sensitive, and convenient test for the detection of antibodies against swine leptospiral infection and can be used as a rapid screening test in epidemiological surveys.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherSpringeres_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceCurrent Microbiology 66 (2) : 106–109 (February 2013)es_AR
dc.subjectEnfermedades de los Animaleses_AR
dc.subjectAnimal Diseaseseng
dc.subjectLeptospirosises_AR
dc.subjectCerdoes_AR
dc.subjectSwineeng
dc.subjectTécnicas Inmunológicases_AR
dc.subjectImmunological Techniqueseng
dc.subjectAntígenoses_AR
dc.subjectAntigenseng
dc.subjectELISAes_AR
dc.subject.otherAnálisis Serológicoes_AR
dc.titleSerological Analysis by Enzyme-Linked Immunosorbent Assay Using Recombinant Antigen LipL32 for the Diagnosis of Swine Leptospirosises_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Patobiologíaes_AR
dc.description.filFil: Hartleben, Cláudia P. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratorio de Imunodiagnóstico; Brasiles_AR
dc.description.filFil: Leal, Fernanda M. A. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratorio de Imunodiagnóstico; Brasiles_AR
dc.description.filFil: Monte, Leonardo G. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratorio de Imunodiagnóstico; Brasiles_AR
dc.description.filFil: Hartwig, Daiane D. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratorio de Vacinologia; Brasiles_AR
dc.description.filFil: Seixas, Fabiana K. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratório de Genômica Funcional; Brasiles_AR
dc.description.filFil: Vasconcellos, Sílvio A. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasiles_AR
dc.description.filFil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentinaes_AR
dc.description.filFil: Dellagostin, Odir A.. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnologia. Laboratorio de Vacinologia; Brasiles_AR
dc.subtypecientifico


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