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Abstract
Genus Helianthus comprises diploid and polyploid species. An autoallopolyploid origin has been proposed for hexaploid species but the genomic relationships remain unclear. Mitotic and meiotic studies in annual Helianthus annuus (2n = 2x = 34) and perennial Helianthus resinosus (2n = 6x = 102) as well as the F1 hybrids between both species were carried out. Chromosome counting confirmed the hybrid origin of the latter plants and their tetraploid condition. [ver mas...]
dc.contributor.authorMiranda Zanetti, Julieta
dc.contributor.authorGreizerstein, Eduardo Jose
dc.contributor.authorCamadro, Elsa Lucila
dc.contributor.authorPoverene, María Mónica
dc.contributor.authorEcheverria, Mercedes
dc.contributor.authorPoggio, Lidia
dc.contributor.authorCarrera, Alicia Delia
dc.date.accessioned2018-12-27T12:45:28Z
dc.date.available2018-12-27T12:45:28Z
dc.date.issued2014-05
dc.identifier.issn0378-2697
dc.identifier.issn1615-6110
dc.identifier.otherhttps://doi.org/10.1007/s00606-013-0945-0
dc.identifier.urihttps://link.springer.com/article/10.1007/s00606-013-0945-0
dc.identifier.urihttp://hdl.handle.net/20.500.12123/4156
dc.description.abstractGenus Helianthus comprises diploid and polyploid species. An autoallopolyploid origin has been proposed for hexaploid species but the genomic relationships remain unclear. Mitotic and meiotic studies in annual Helianthus annuus (2n = 2x = 34) and perennial Helianthus resinosus (2n = 6x = 102) as well as the F1 hybrids between both species were carried out. Chromosome counting confirmed the hybrid origin of the latter plants and their tetraploid condition. Bivalents in hybrids ranged from 12 to 28 ( x¯ = 20.8). Univalents, trivalents and quadrivalents were also observed. Meiotic products comprised dyads, triads and normal tetrads and pollen grains were heterogeneous in size. These observations suggest the occurrence of 2n pollen in addition to the expected n. Genomic in situ hybridization (GISH) of total H. annuus DNA on H. resinosus chromosomes rendered weak but uniform signals; similar hybridization pattern was observed using three other annual species. Hybridization with H. annuus probe performed on root tip cells of F1 H. annuus × H. resinosus hybrids revealed 17 chromosomes with a strong hybridization signal. GISH in hybrid meiocytes distinguished chromosomes from parental species and revealed autosyndetic pairing of H. resinosus chromosomes, allosyndetic pairing in bivalents, trivalents and quadrivalents, and the presence of univalents derived from parents, H. annuus and H. resinosus. Results obtained from classical and molecular cytogenetics do not support H. annuus as a direct ancestor of H. resinosus. The occurrence of allosyndetic pairing and the relatively high fertility of the F1 hybrids point to the possibility that useful genes could be transferred from H. resinosus to cultivate sunflower, although the effective rate of recombination has not been evaluated. GISH method proved effective to recognize parental chromosomes in H. annuus × H. resinosus progeny.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherSpringeres_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourcePlant Systematics and Evolution 300 (5) : 1071–1078 (May 2014)es_AR
dc.subjectHelianthuses_AR
dc.subjectHelianthus Annuuses_AR
dc.subjectHexaploidiaes_AR
dc.subjectHexaploidyeng
dc.subjectDiploidiaes_AR
dc.subjectDiploidyeng
dc.subjectGenómicaes_AR
dc.subjectGenomicseng
dc.subjectPoliploidiaes_AR
dc.subjectPolyploidyeng
dc.subjectHibridación Interespecíficaes_AR
dc.subjectInterspecific Hybridizationeng
dc.subject.otherHelianthus resinosuses_AR
dc.subject.otherGirasoles_AR
dc.titleGenomic relationships between hexaploid Helianthus resinosus and diploid Helianthus annuus (Asteraceae)es_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEEA Balcarcees_AR
dc.description.filFil: Miranda Zanetti, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentinaes_AR
dc.description.filFil: Greizerstein, Eduardo Jose. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Universidad Nacional de Lomas de Zamora. Facultad de Ciencias Agrarias; Argentinaes_AR
dc.description.filFil: Camadro, Elsa Lucila. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Poverene, María Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentinaes_AR
dc.description.filFil: Echeverria, Mercedes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentinaes_AR
dc.description.filFil: Poggio, Lidia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Carrera, Alicia Delia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentinaes_AR
dc.subtypecientifico


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