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Resumen
Whole genome sequencing of a copper resistant (CuR) black rot strain of Xanthomonas campestris pv. campestris (Xcc) isolated from a broccoli plant in Trinidad revealed a unique operon for copper resistance. The cop genes of strain Xcc-BrA1 were determined to be present on a 160 to 180 kb plasmid shown to be non-conjugative with other xanthomonads. While nucleotide comparison of a putative 8.0 Kbp copLABMGF gene cluster identified in Xcc-BrA1 genome did [ver mas...]
dc.contributor.authorBehlau, Franklin
dc.contributor.authorGochez, Alberto Martin
dc.contributor.authorLugo, Alshia Janel
dc.contributor.authorElibox, Winston
dc.contributor.authorMinsavage, Gerald V.
dc.contributor.authorPotnis, Neha
dc.contributor.authorWhite, Frank F.
dc.contributor.authorEbrahim, Mohamed
dc.contributor.authorJones, Jeffrey B.
dc.contributor.authorRamsubhag, Adesh
dc.date.accessioned2018-12-26T16:54:48Z
dc.date.available2018-12-26T16:54:48Z
dc.date.issued2017-03
dc.identifier.issn0929-1873
dc.identifier.issn1573-8469
dc.identifier.otherhttps://doi.org/10.1007/s10658-016-1035-2
dc.identifier.urihttps://link.springer.com/article/10.1007/s10658-016-1035-2
dc.identifier.urihttp://hdl.handle.net/20.500.12123/4150
dc.description.abstractWhole genome sequencing of a copper resistant (CuR) black rot strain of Xanthomonas campestris pv. campestris (Xcc) isolated from a broccoli plant in Trinidad revealed a unique operon for copper resistance. The cop genes of strain Xcc-BrA1 were determined to be present on a 160 to 180 kb plasmid shown to be non-conjugative with other xanthomonads. While nucleotide comparison of a putative 8.0 Kbp copLABMGF gene cluster identified in Xcc-BrA1 genome did not reveal any homologous region with other known CuR Xanthomonas strains from diverse origins, the comparison of the translated amino acid sequence indicated similarity with X. citri, X. c. pv. citrumelonis and X. vesicatoria Cop proteins. Cloning of the copLAB gene cluster from Xcc-BrA1 conferred copper resistance to other copper-sensitive xanthomonads. Although Xcc-BrA1 harbors copLAB genes with similar sizes and organization and is able to grow on Cu-amended medium as other CuR xanthomonads, the phylogenetic analysis of nucleotide sequences indicates that the cop cluster in Xcc-BrA1 is unique and distantly related to other copLAB genes from Xanthomonas and Stenotrophomonas. The origin of copper resistance genes in Xcc-BrA1 is likely a result of horizontal gene acquisition from a still unknown phylloplane cohabitant. The findings of this study have implications for the management of crop diseases caused by CuR xanthomonads. Future studies could focus on and determining the distribution, overall importance and appropriate control measures for strains harbouring these unique genes.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherSpringeres_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceEuropean Journal of Plant Pathology 147 (3) : 671–681 (March 2017)es_AR
dc.subjectXanthomonas campestrises_AR
dc.subjectGenéticaes_AR
dc.subjectGeneticseng
dc.subjectEnfermedades de las Plantases_AR
dc.subjectPlant Diseaseseng
dc.subjectCobrees_AR
dc.subjectCoppereng
dc.subjectFilogeniaes_AR
dc.subjectPhylogenyeng
dc.subjectGenomases_AR
dc.subjectGenomeseng
dc.subject.otherTrinidad y Tobagoes_AR
dc.subject.otherXanthomonas campestris pv. campestris
dc.titleCharacterization of a unique copper resistance gene cluster in Xanthomonas campestris pv. campestris isolated in Trinidad, West Indieses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEEA Bella Vistaes_AR
dc.description.filFil: Behlau, Franklin. Fundo de Defesa da Citricultura. Pesquisa e Desenvolvimento; Brasiles_AR
dc.description.filFil: Gochez, Alberto Martin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bella Vista; Argentinaes_AR
dc.description.filFil: Lugo, Alshia Janel. University of the West Indies. Faculty of Science and Technology. Department of Life Sciences; Trinidad y Tobagoes_AR
dc.description.filFil: Elibox, Winston. University of the West Indies. Faculty of Science and Technology. Department of Life Sciences; Trinidad y Tobagoes_AR
dc.description.filFil: Minsavage, Gerald V. University of Florida. Department of Plant Pathology; Estados Unidoses_AR
dc.description.filFil: Potnis, Neha . University of Florida. Department of Plant Pathology; Estados Unidoses_AR
dc.description.filFil: White, Frank F. Kansas State University. Department of Plant Pathology; Estados Unidoses_AR
dc.description.filFil: Ebrahim, Mohamed. University of Florida. Department of Plant Pathology; Estados Unidos. Ain Shams University. Faculty of Agriculture. Department of Plant Pathology; Egiptoes_AR
dc.description.filFil: Jones, Jeffrey B. University of Florida. Department of Plant Pathology; Estados Unidoses_AR
dc.description.filFil: Ramsubhag, Adesh. University of the West Indies. Faculty of Science and Technology. Department of Life Sciences; Trinidad y Tobagoes_AR
dc.subtypecientifico


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