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Resumen
We have determined the full-length 14,491-nucleotide genome sequence of a new plant rhabdovirus, alfalfa dwarf virus (ADV). Seven open reading frames (ORFs) were identified in the antigenomic orientation of the negative-sense, single-stranded viral RNA, in the order 3′-N-P-P3-M-G-P6-L-5′. The ORFs are separated by conserved intergenic regions and the genome coding region is flanked by complementary 3′ leader and 5′ trailer sequences. Phylogenetic analysis [ver mas...]
dc.contributor.authorBejerman, Nicolas Esteban
dc.contributor.authorGiolitti, Fabian
dc.contributor.authorDe Breuil, Soledad
dc.contributor.authorTrucco, Veronica Milagros
dc.contributor.authorNome Docampo, Claudia
dc.contributor.authorLenardon, Sergio Luis
dc.contributor.authorDietzgen, Ralf Georg
dc.date.accessioned2018-11-06T18:04:21Z
dc.date.available2018-11-06T18:04:21Z
dc.date.issued2015-09
dc.identifier.issn0042-6822
dc.identifier.otherhttps://doi.org/10.1016/j.virol.2015.05.001
dc.identifier.urihttp://hdl.handle.net/20.500.12123/3803
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0042682215002457?via%3Dihub
dc.description.abstractWe have determined the full-length 14,491-nucleotide genome sequence of a new plant rhabdovirus, alfalfa dwarf virus (ADV). Seven open reading frames (ORFs) were identified in the antigenomic orientation of the negative-sense, single-stranded viral RNA, in the order 3′-N-P-P3-M-G-P6-L-5′. The ORFs are separated by conserved intergenic regions and the genome coding region is flanked by complementary 3′ leader and 5′ trailer sequences. Phylogenetic analysis of the nucleoprotein amino acid sequence indicated that this alfalfa-infecting rhabdovirus is related to viruses in the genus Cytorhabdovirus. When transiently expressed as GFP fusions in Nicotiana benthamiana leaves, most ADV proteins accumulated in the cell periphery, but unexpectedly P protein was localized exclusively in the nucleus. ADV P protein was shown to have a homotypic, and heterotypic nuclear interactions with N, P3 and M proteins by bimolecular fluorescence complementation. ADV appears unique in that it combines properties of both cytoplasmic and nuclear plant rhabdoviruses.eng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.publisherElsevieres_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.sourceVirology 483 : 275-283. (September 2015)eng
dc.subjectMedicago Sativaes_AR
dc.subjectGenomases_AR
dc.subjectGenomeseng
dc.subjectCytorhabdoviruses_AR
dc.subjectNucleorhabdoviruses_AR
dc.subjectProteinseng
dc.subjectProteínases_AR
dc.subjectPhosphoproteinseng
dc.subjectFosfoproteínases_AR
dc.subject.otherAlfalfaes_AR
dc.subject.otherRabdoviruses_AR
dc.titleComplete genome sequence and integrated protein localization and interaction map for alfalfa dwarf virus, which combines properties of both cytoplasmic and nuclear plant rhabdoviruseseng
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenInstituto de Patología Vegetales_AR
dc.description.filFil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; Australiaes_AR
dc.description.filFil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentinaes_AR
dc.description.filFil: De Breuil, Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Trucco, Veronica Milagros: Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentinaes_AR
dc.description.filFil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentinaes_AR
dc.description.filFil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentinaes_AR
dc.description.filFil: Dietzgen, Ralf Georg. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; Australiaes_AR
dc.subtypecientifico


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