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Abstract
Sunflower chlorotic mottle virus (SuCMoV), the most prevalent virus of sunflower in Argentina, was reported naturally infecting not only sunflower but also weeds. To understand SuCMoV evolution and improve the knowledge on its variability, the complete genomic sequences of two SuCMoV isolates collected from Dipsacus fullonum (-dip) and Ibicella lutea (-ibi) were determined from three overlapping cDNA clones and subjected to phylogenetic and recombination [ver mas...]
dc.contributor.authorBejerman, Nicolas Esteban
dc.contributor.authorGiolitti, Fabian
dc.contributor.authorDe Breuil, Soledad
dc.contributor.authorLenardon, Sergio Luis
dc.date.accessioned2018-11-05T12:32:39Z
dc.date.available2018-11-05T12:32:39Z
dc.date.issued2013-02
dc.identifier.issn0920-8569
dc.identifier.issn1572-994X (Online)
dc.identifier.urihttp://hdl.handle.net/20.500.12123/3778
dc.description.abstractSunflower chlorotic mottle virus (SuCMoV), the most prevalent virus of sunflower in Argentina, was reported naturally infecting not only sunflower but also weeds. To understand SuCMoV evolution and improve the knowledge on its variability, the complete genomic sequences of two SuCMoV isolates collected from Dipsacus fullonum (-dip) and Ibicella lutea (-ibi) were determined from three overlapping cDNA clones and subjected to phylogenetic and recombination analyses. SuCMoV-dip and -ibi genomes were 9,953-nucleotides (nt) long; their sequences contained an open reading frame of 9,561 nucleotides, which encoded a polyprotein of 3,187 amino acids flanked by a 5′-noncoding region (NCR) of 135 nt and a 3′-NCR of 257 nt. SuCMoV-dip and -ibi genome nucleotide sequences were 90.9 identical and displayed 90 and 94.6 % identity to that of SuCMoV-C, and 90.8 and 91.4 % identity to that of SuCMoV-CRS, respectively. P1 of SuCMoV-dip and -ibi was 3-nt longer than that of SuCMoV-CRS, but 12-nt shorter than that of SuCMoV-C. Two recombination events were detected in SuCMoV genome and the analysis of dN/dS ratio among SuCMoV complete sequences showed that the genomic regions are under different evolutionary constraints, suggesting that SuCMoV evolution would be conservative. Our findings provide evidence that mutation and recombination would have played important roles in the evolutionary history of SuCMoV.eng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.publisherSpringereng
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.sourceVirus genes 46 (1) : 105–110. (February 2013)eng
dc.subjectHelianthus Annuuses_AR
dc.subjectPotyviruses_AR
dc.subjectPotyviruseseng
dc.subjectGenomeseng
dc.subjectGenomases_AR
dc.subject.otherGirasoles_AR
dc.subject.otherSunflowereng
dc.subject.otherSecuenciaciónes_AR
dc.subject.otherSequencingeng
dc.subject.otherPhylogenetic Analysiseng
dc.subject.otherAnálisis Filogenéticoes_AR
dc.subject.otherSuCMoVeng
dc.titleSequencing of two Sunflower chlorotic mottle virus isolates obtained from different natural hosts shed light on its evolutionary historyeng
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenInstituto de Patología Vegetales_AR
dc.description.filFil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentinaes_AR
dc.description.filFil: De Breuil, Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentinaes_AR
dc.subtypecientifico


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