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Abstract
Rust fungi are one of the most devastating pathogens of crop plants. The biotrophic fungus Puccinia sorghi Schwein (Ps) is responsible for maize common rust, an endemic disease of maize (Zea mays L.) in Argentina that causes significant yield losses in corn production. In spite of this, the Ps genomic sequence was not available. We used Illumina sequencing to rapidly produce the 99.6 Mb draft genome sequence of Ps race RO10H11247, derived from a [ver mas...]
dc.contributor.authorRochi, Lucía
dc.contributor.authorDieguez, Maria Jose
dc.contributor.authorBurguener, Germán
dc.contributor.authorDarino, Martín Alejandro
dc.contributor.authorPergolesi, María Fernanda
dc.contributor.authorIngala, Lorena Romina
dc.contributor.authorCuyeu, Alba Romina
dc.contributor.authorTurjanski, Adrián
dc.contributor.authorKreff, Enrique D.
dc.contributor.authorSacco, Francisco
dc.date.accessioned2018-10-24T17:23:29Z
dc.date.available2018-10-24T17:23:29Z
dc.date.issued2018-03
dc.identifier.issn1087-1845
dc.identifier.otherhttp://dx.doi.org/10.1016/j.fgb.2016.10.001
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S1087184516301098?via%3Dihub
dc.identifier.urihttp://hdl.handle.net/20.500.12123/3698
dc.description.abstractRust fungi are one of the most devastating pathogens of crop plants. The biotrophic fungus Puccinia sorghi Schwein (Ps) is responsible for maize common rust, an endemic disease of maize (Zea mays L.) in Argentina that causes significant yield losses in corn production. In spite of this, the Ps genomic sequence was not available. We used Illumina sequencing to rapidly produce the 99.6 Mb draft genome sequence of Ps race RO10H11247, derived from a single-uredinial isolate from infected maize leaves collected in the Argentine Corn Belt Region during 2010. High quality reads were obtained from 200 bp paired-end and 5000 bp mate-paired libraries and assembled in 15,722 scaffolds. A pipeline which combined an ab initio program with homology-based models and homology to in planta enriched ESTs from four cereal pathogenic fungus (the three sequenced wheat rusts and Ustilago maydis) was used to identify 21,087 putative coding sequences, of which 1599 might be part of the Ps RO10H11247 secretome. Among the 458 highly conserved protein families from the euKaryotic Orthologous Groups (KOG) that occur in a wide range of eukaryotic organisms, 97.5% have at least one member with high homology in the Ps assembly (TBlastN, E-value ⩽ e−10) covering more than 50% of the length of the KOG protein. Comparative studies with the three sequenced wheat rust fungus, and microsynteny analysis involving Puccinia striiformis f. sp. tritici (Pst, wheat stripe rust fungus), support the quality achieved. The results presented here show the effectiveness of the Illumina strategy for sequencing dikaryotic genomes of non-model organisms and provides reliable DNA sequence information for genomic studies, including pathogenic mechanisms of this maize fungus and molecular marker design.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherElsevieres_AR
dc.relationinfo:eu-repograntAgreement/INTA/PNBIO/1131042/AR./Genómica aplicada al mejoramiento molecular.es_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceFungal Genetics and Biology 112 : 31-39 (Marzo 2018)es_AR
dc.subjectGenetic mapseng
dc.subjectMapas Genéticoses_AR
dc.subjectMaizeeng
dc.subjectMaízes_AR
dc.subjectZea mayses_AR
dc.subjectPuccinia sorghies_AR
dc.subjectPathogenseng
dc.subjectOrganismos patógenoses_AR
dc.subject.otherGenome analysiseng
dc.subject.otherAnálisis genómicoes_AR
dc.titleCharacterization and comparative analysis of the genome of Puccinia sorghi Schwein, the causal agent of maize common rustes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Genéticaes_AR
dc.description.filFil: Rochi, Lucía. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentinaes_AR
dc.description.filFil: Dieguez, María José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentinaes_AR
dc.description.filFil: Burguener, Germán. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentinaes_AR
dc.description.filFil: Darino, Martín Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentinaes_AR
dc.description.filFil: Pergolesi, Marí­a Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentinaes_AR
dc.description.filFil: Ingala, Lorena Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentinaes_AR
dc.description.filFil: Cuyeu, Alba Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentinaes_AR
dc.description.filFil: Turjanski, Adrián. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentinaes_AR
dc.description.filFil: Kreff, Enrique D. Pioneer Hi-Bred International; Argentinaes_AR
dc.description.filFil: Sacco, Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentinaes_AR
dc.subtypecientifico


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