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Abstract
Alfalfa (Medicago sativa L.) is the main forage crop in Argentina and plays an essential role in the animal production chain, but it can be negatively affected by many factors, such as viral diseases. Here, we report the presence of Bean leafroll virus infecting alfalfa (BLRV-Arg), its distribution on alfalfa fields and its complete genome sequence. BLRV-Arg showed a prevalence of over 50 % and a wide distribution in Argentine alfalfa fields. The BLRV [ver mas...]
dc.contributor.authorTrucco, Veronica Milagros
dc.contributor.authorDe Breuil, Soledad
dc.contributor.authorBejerman, Nicolas Esteban
dc.contributor.authorLenardon, Sergio Luis
dc.contributor.authorGiolitti, Fabian
dc.date.accessioned2018-10-22T11:34:28Z
dc.date.available2018-10-22T11:34:28Z
dc.date.issued2016-09
dc.identifier.issn0929-1873
dc.identifier.otherhttps://doi.org/10.1007/s10658-016-0899-5
dc.identifier.urihttp://hdl.handle.net/20.500.12123/3655
dc.identifier.urihttps://link.springer.com/article/10.1007%2Fs10658-016-0899-5
dc.description.abstractAlfalfa (Medicago sativa L.) is the main forage crop in Argentina and plays an essential role in the animal production chain, but it can be negatively affected by many factors, such as viral diseases. Here, we report the presence of Bean leafroll virus infecting alfalfa (BLRV-Arg), its distribution on alfalfa fields and its complete genome sequence. BLRV-Arg showed a prevalence of over 50 % and a wide distribution in Argentine alfalfa fields. The BLRV genome of the Manfredi isolate (Manfredi BLRV-Arg) is 5884 nucleotides in length, with a genome organization similar to that already reported for this virus. The complete nucleotide sequence or selected genes were subjected to sequence comparisons. Manfredi BLRV-Arg had more than 96 % nucleotide and amino acid sequence identities, both for complete genome sequence and ORFs, with those of the two full-length genome sequences available in GenBank. The complete CP sequences of Manfredi BLRV-Arg showed an identity percentage over 98 % at nt and deduced aa level when were compared with those of the other CP complete sequences available in GenBank. This is the first evidence for the presence of BLRV in Argentina, its geographical distribution in alfalfa production areas and the complete genome sequence of an isolate from alfalfa as natural host.eng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.publisherSpringereng
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.sourceEuropean journal of plant pathology 146 (1) : 207–212. (September 2016)eng
dc.subjectMedicago Sativaes_AR
dc.subjectLuteoviruses_AR
dc.subjectBean Leafroll Viruseng
dc.subjectGenomases_AR
dc.subjectGenomeseng
dc.subjectDistribución Geográficaes_AR
dc.subjectGeographical Distributioneng
dc.subject.otherAlfalfaes_AR
dc.subject.otherArgentinaes_AR
dc.subject.otherBLRVeng
dc.subject.otherSecuenciaciónes_AR
dc.subject.otherSequencingeng
dc.subject.otherField Detectioneng
dc.titleBean leafroll virus (BLRV) in Argentina: molecular characterization and detection in alfalfa fieldseng
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenInstituto de Patología Vegetales_AR
dc.description.filFil: Trucco, Veronica Milagros. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentinaes_AR
dc.description.filFil: De Breuil, Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentinaes_AR
dc.description.filFil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentinaes_AR
dc.subtypecientifico


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