Show simple item record

resumen

Abstract
We used transient expression assays to assess the function of the baculovirus Spodoptera frugiperda M nucleopolyhedrovirus (SfMNPV) homologs of Autographa californica MNPV (AcMNPV) factors involved in late gene expression (lefs), in the Sf9 insect cell-line, which is permissive for both viruses. It is well-established that nineteen AcMNPV lefs support optimal levels of activity from a late promoter-reporter gene cassette in this assay. A subgroup of [ver mas...]
dc.contributor.authorBerretta, Marcelo Facundo
dc.contributor.authorLopez, Maria Gabriela
dc.contributor.authorTaboga, Oscar Alberto
dc.contributor.authorSciocco, Alicia Ines
dc.contributor.authorRomanowski, Victor
dc.date.accessioned2018-10-16T12:55:12Z
dc.date.available2018-10-16T12:55:12Z
dc.date.issued2013-02
dc.identifier.issn0920-8569
dc.identifier.issn1572-994X
dc.identifier.otherhttps://doi.org/10.1007/s11262-012-0843-5
dc.identifier.urihttps://link.springer.com/article/10.1007%2Fs11262-012-0843-5
dc.identifier.urihttp://hdl.handle.net/20.500.12123/3598
dc.description.abstractWe used transient expression assays to assess the function of the baculovirus Spodoptera frugiperda M nucleopolyhedrovirus (SfMNPV) homologs of Autographa californica MNPV (AcMNPV) factors involved in late gene expression (lefs), in the Sf9 insect cell-line, which is permissive for both viruses. It is well-established that nineteen AcMNPV lefs support optimal levels of activity from a late promoter-reporter gene cassette in this assay. A subgroup of SfMNPV lefs predicted to function in transcription-specific events substituted the corresponding AcMNPV lefs very efficiently. When all SfMNPV lefs were assayed, including replication lefs, activity was low, but addition of two AcMNPV lefs not encoded in SfMNPV genome, resulted in augmented reporter activity. SfMNPV IE-1 was able to activate an early promoter cis-linked to an hr-derived element from SfMNPV but not from AcMNPV. However, the level of early promoter activation with SfMNPV IE-1 was lower compared to AcMNPV IE-1.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceVirus Genes 46 (1) : 152–161 (February 2013)es_AR
dc.subjectSpodoptera frugiperdaes_AR
dc.subjectLepidopteraes_AR
dc.subjectExpresión Génicaes_AR
dc.subjectGene Expressioneng
dc.subjectBaculoviruses_AR
dc.subject.otherNucleopolyhedroviruses_AR
dc.subject.otherSf9 Cellseng
dc.titleFunctional analysis of Spodoptera frugiperda nucleopolyhedrovirus late expression factors in Sf9 cellses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Microbiología y Zoología Agrícolaes_AR
dc.description.filFil: Berretta, Marcelo Facundo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentinaes_AR
dc.description.filFil: Lopez, Maria Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina.es_AR
dc.description.filFil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Sciocco, Alicia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentinaes_AR
dc.description.filFil: Romanowski, Victor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentinaes_AR
dc.subtypecientifico


Files in this item

Thumbnail

This item appears in the following Collection(s)

common

Show simple item record