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Abstract
Somatic embryogenesis in scutella of wheat (Triticum aestivum L.) is a well documented phenomenon and it has been shown through transcriptome analysis that genes involved in antioxidant responses, particularly in glutathione (GSH) biosynthesis, participate in the process. Thus, we investigated the influence of post-transcriptional silencing (PTGS) of the glutathione biosynthesis genes GSH1 and GSH2 on somatic embryogenesis in wheat. We found that PTGS of [ver mas...]
dc.contributor.authorBossio, Adrian Ezequiel
dc.contributor.authorDiaz Paleo, Antonio Horacio
dc.contributor.authorDel Vas, Mariana
dc.contributor.authorBaroli, Irene
dc.contributor.authorAcevedo, Alberto
dc.contributor.authorRios, Raul Daniel
dc.date.accessioned2018-09-20T17:25:42Z
dc.date.available2018-09-20T17:25:42Z
dc.date.issued2013-02
dc.identifier.issn1573-5044
dc.identifier.otherhttps://doi.org/10.1007/s11240-012-0228-4
dc.identifier.urihttp://hdl.handle.net/20.500.12123/3432
dc.identifier.urihttps://link.springer.com/article/10.1007/s11240-012-0228-4
dc.description.abstractSomatic embryogenesis in scutella of wheat (Triticum aestivum L.) is a well documented phenomenon and it has been shown through transcriptome analysis that genes involved in antioxidant responses, particularly in glutathione (GSH) biosynthesis, participate in the process. Thus, we investigated the influence of post-transcriptional silencing (PTGS) of the glutathione biosynthesis genes GSH1 and GSH2 on somatic embryogenesis in wheat. We found that PTGS of either of the target genes drastically inhibits callus regeneration and overall efficiency of transformation, in a similar manner as the GSH biosynthetic inhibitor buthionine sulfoximine. Supplementing the medium with glutathione did not overcome the observed low efficiency of wheat transformation. Furthermore, of the small number of obtained transformants, none exhibited altered GSH1 and GSH2 levels of transcription. Thus, it is concluded that GSH is essential for somatic embryogenesis and, as a consequence, it is difficult to regenerate wheat plants with silenced GSH1 and GSH2 genes.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherSpringeres_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourcePlant Cell, Tissue and Organ Culture 112 (2) : 239–248 (February 2013)es_AR
dc.subjectEmbriogénesis Somáticaes_AR
dc.subjectSomatic Embryogenesiseng
dc.subjectTrigoes_AR
dc.subjectWheateng
dc.subjectTriticum aestivumes_AR
dc.subjectBiosíntesises_AR
dc.subjectBioshyntesiseng
dc.subjectGlutationes_AR
dc.subjectGlutathioneeng
dc.subjectTranscripción Génicaes_AR
dc.subjectGene Transcriptioneng
dc.subject.otherSilenciamiento postranscripcionales_AR
dc.titleSilencing of the glutathione biosynthetic pathway inhibits somatic embryogenesis in wheates_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Genéticaes_AR
dc.description.filFil: Bossio, Adrian Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentinaes_AR
dc.description.filFil: Diaz Paleo, Antonio Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentinaes_AR
dc.description.filFil: Del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina.es_AR
dc.description.filFil: Baroli, Irene. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Acevedo, Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación de Recursos Naturales; Argentinaes_AR
dc.description.filFil: Ríos, Raúl Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentinaes_AR
dc.subtypecientifico


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