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Cold sweetening diversity in Andean potato germplasm from Argentina
Colman, Silvana; Massa, Gabriela Alejandra; Carboni, Martín Federico; Feingold, Sergio Enrique (Wiley Online Library, 2018-04-02)BACKGROUND: Cold induced sweetening (CIS) is the accumulation of sucrose and reducing sugars in potato tubers at low temperatures. This process is central for the potato processing industry. During potato chip and French ... -
Comparative potato genome editing: Agrobacterium tumefaciens-mediated transformation and protoplasts transfection delivery of CRISPR/Cas9 components directed to StPPO2 gene
Gonzalez, Matías Nicolás; Massa, Gabriela Alejandra; Anderson, Mariette; Decima Oneto, Cecilia Andrea; Turesson, Helle; Olsson, Niklas; Fält, Ann Sofie; Hofvander, Per; Feingold, Sergio Enrique (Springer Netherlands, 2021-01-25)Delivery of the CRISPR/Cas9 components to the plant cells is a key step in its application as a genome editing tool. Here, we compared Agrobacterium-mediated transformation and protoplast transfection with CRISPR/Cas9 ... -
Edición génica con el sistema CRISPR/Cas9: historia de su descubrimiento y alcances en agricultura
Gonzalez, Matías Nicolás; Massa, Gabriela Alejandra; Feingold, Sergio Enrique (Universidad de Los Andes, Venezuela, 2021-04)La conversión del sistema inmune procariota CRISPR/Cas9 en una herramienta molecular para edición génica representa el avance tecnológico más destacado de la última década, y ha revolucionado tanto la investigación básica ... -
Efficient Agrobacterium tumefaciens-mediated transformation system of Diaporthe caulivora
Montoya, Marina Rosa; Massa, Gabriela Alejandra; Colabelli, Mabel Noemí; Ridao, Azucena (Elsevier, 2021-03-10)This is the first report describing the genetic transformation of Diaporthe caulivora, the soybean stem canker fungus. A simple and 100% efficient protocol of Agrobacterium tumefaciens-mediated transformation used mycelium ... -
Evidence of Recombinant Isolates of Potato Virus Y (PVY) in Argentina
Colavita, Monica; Lancia, Marcos; Feingold, Sergio Enrique; Massa, Gabriela Alejandra (Springer Netherlands, 2017-06)In order to make a first approach in the identification of the genetic diversity of Potato virus Y (PVY) in Argentina, 46 PVY isolates from different potato growing regions of Argentina were characterized both, biological ... -
Genome-Wide Analyses of Aspartic Proteases on Potato Genome (Solanum tuberosum): Generating New Tools to Improve the Resistance of Plants to Abiotic Stress
Norero, Natalia Sigrid; Rey Burusco, María Florencia; D’Ippólito, Sebastián; Decima Oneto, Cecilia Andrea; Massa, Gabriela Alejandra; Castellote, Martín Alfredo; Feingold, Sergio Enrique; Guevara, María Gabriela (Multidisciplinary Digital Publishing Institute, MDPI, 2022-02)Aspartic proteases are proteolytic enzymes widely distributed in living organisms and viruses. Although they have been extensively studied in many plant species, they are poorly described in potatoes. The present study ... -
Reduced enzymatic browning in potato tubers by specific editing of a Polyphenol oxidase gene via Ribonucleoprotein complexes delivery of the CRISPR/Cas9 System.
Gonzalez, Matías Nicolás; Massa, Gabriela Alejandra; Andersson, Mariette; Turesson, Helle; Olsson, Niklas; Fält, Ann-Sofie; Storani, Leonardo; Decima Oneto, Cecilia Andrea; Hofvander, Per; Feingold, Sergio Enrique (Frontiers Media, Switzerland, 2020-01-09)Polyphenol Oxidases (PPOs) catalyze the conversion of phenolic substrates to quinones, leading to the formation of dark-colored precipitates in fruits and vegetables. This process, known as enzymatic browning, is the cause ... -
Transformación eficiente de Solanum tuberosum cv. Spunta mediada por Agrobacterium tumefaciens utilizando higromicina como agente selectivo
Decima Oneto, Cecilia Andrea; Coronel, Joel; Storani, Leonardo; Gonzalez, Matías Nicolás; Feingold, Sergio Enrique; Massa, Gabriela Alejandra (Ediciones INTA, 2020-08)La transformación genética de plantas es una herramienta biotecnológica de alto impacto en el mejoramiento vegetal que brinda la posibilidad de obtener nuevos cultivos que se adapten a las demandas de producción y de ...