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Abstract
In previous studies, the applicability of polymeric hydrogels for the protection of egg yolk immunoglobulin (IgY) against simulated gastric conditions was established. Thereafter, the performance of the hydrogels was improved with the addition of chitosan wrapped carbon nanotubes and the in vitro toxicity for porcine intestinal cells of these nanocomposites was assessed. The objective of the present study was to evaluate in vivo the protective efficacy of [ver mas...]
dc.contributor.authorAlustiza, Fabrisio Eduardo
dc.contributor.authorBellingeri, Romina
dc.contributor.authorPicco, Natalia
dc.contributor.authorMotta, Carlos
dc.contributor.authorGrosso, M. Carolina
dc.contributor.authorBarbero, Cesar A.
dc.contributor.authorAcevedo, Diego F.
dc.contributor.authorVivas, Adriana
dc.date.accessioned2018-07-27T14:55:16Z
dc.date.available2018-07-27T14:55:16Z
dc.date.issued2016-06-14
dc.identifier.issn0264-410X
dc.identifier.otherhttps://doi.org/10.1016/j.vaccine.2016.05.004
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0264410X16302766?via%3Dihub
dc.identifier.urihttp://hdl.handle.net/20.500.12123/2904
dc.description.abstractIn previous studies, the applicability of polymeric hydrogels for the protection of egg yolk immunoglobulin (IgY) against simulated gastric conditions was established. Thereafter, the performance of the hydrogels was improved with the addition of chitosan wrapped carbon nanotubes and the in vitro toxicity for porcine intestinal cells of these nanocomposites was assessed. The objective of the present study was to evaluate in vivo the protective efficacy of the nanocomoposite matrix for IgY when the immunoglobulin is used against enterotoxigenic Escherichia coli (ETEC) in challenged piglets. Groups of piglets orally challenged with 1011 CFU/mL of ETEC were treated with non-protected and protected IgY. The clinical response of each group was monitored and evaluated in terms of dehydration, rectal temperature, faecal consistency score and body weight gain. Blood parameters and histological aspects were also studied. The results showed that treatment of infected piglets with protected IgY reduced significantly the severity of diarrhea. Non-protected IgY group show a lower recovery rate. Blood parameters and histological aspects were normal in both groups. Collectively, these results support previous in vitro studies showing that the nanocomposites can be an effective method of IgY protection against gastric inactivation.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceVaccine 34 (28) : 3291-3297 (June 2016)es_AR
dc.subjectInmunoglobulinaes_AR
dc.subjectImmunoglobulinseng
dc.subjectYema de Huevoes_AR
dc.subjectEgg Yolkeng
dc.subjectEscherichia colies_AR
dc.subjectGeleses_AR
dc.subjectGelseng
dc.subjectNanocompuestoses_AR
dc.subjectNanocompositeseng
dc.subjectLechónes_AR
dc.subjectPigletseng
dc.subjectPrevención de Enfermedadeses_AR
dc.subjectDisease Preventioneng
dc.subjectDiarreaes_AR
dc.subjectDiarrhoeaeng
dc.titleIgY against enterotoxigenic Escherichia coli administered by hydrogel-carbon nanotubes composites to prevent neonatal diarrhoea in experimentally challenged pigletses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEEA Marcos Juárezes_AR
dc.description.filFil: Alustiza, Fabrisio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentinaes_AR
dc.description.filFil: Bellingeri, Romina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal. Laboratorio de Biotecnología Animal; Argentinaes_AR
dc.description.filFil: Picco, Natalia. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal. Laboratorio de Biotecnología Animal; Argentinaes_AR
dc.description.filFil: Motta, Carlos. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Patología Animal; Argentinaes_AR
dc.description.filFil: Grosso, M. Carolina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal. Laboratorio de Biotecnología Animal; Argentinaes_AR
dc.description.filFil: Barbero, Cesar A.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Química. Laboratorio de Materiales Avanzados; Argentinaes_AR
dc.description.filFil: Acevedo, Diego F. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Química. Laboratorio de Materiales Avanzados; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ingeniería. Departamento de Tecnología Química; Argentinaes_AR
dc.description.filFil: Vivas, Adriana. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal. Laboratorio de Biotecnología Animal; Argentinaes_AR
dc.subtypecientifico


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