Mostrar el registro sencillo del ítem

resumen

Resumen
Light, electron microscopy and DNA analyses were performed to characterize a microsporidium infecting Rachiplusia nu larvae from a laboratory rearing in Argentina. Diplokaryotic spores were oval and measured 3.61 ± 0.29 × 1.61 ± 0.14 μM (fresh). The spore wall was composed of an electron-dense exospore and an electron-lucent endospore, ca. 30 nm and 100–120 nm thick, respectively. The polar filament was arranged in a single rank of 10–12 coils (typically [ver mas...]
dc.contributor.authorArneodo Larochette, Joel Demian
dc.contributor.authorSciocco, Alicia Ines
dc.date.accessioned2018-07-24T18:15:19Z
dc.date.available2018-07-24T18:15:19Z
dc.date.issued2018-05
dc.identifier.issn0932-0113
dc.identifier.issn1432-1955 (Online)
dc.identifier.otherhttps://doi.org/10.1007/s00436-018-5820-z
dc.identifier.urihttp://hdl.handle.net/20.500.12123/2866
dc.identifier.urihttps://link.springer.com/article/10.1007%2Fs00436-018-5820-z
dc.description.abstractLight, electron microscopy and DNA analyses were performed to characterize a microsporidium infecting Rachiplusia nu larvae from a laboratory rearing in Argentina. Diplokaryotic spores were oval and measured 3.61 ± 0.29 × 1.61 ± 0.14 μM (fresh). The spore wall was composed of an electron-dense exospore and an electron-lucent endospore, ca. 30 nm and 100–120 nm thick, respectively. The polar filament was arranged in a single rank of 10–12 coils (typically 11). Microsporidian cells were found in the cytoplasm, next to the endoplasmic reticulum (especially the prespore stages) and generally surrounded by electron-lucent spaces. The infection was polyorganotropic; the fat body appeared as the most heavily invaded tissue, followed by tracheal matrix and epidermis. A molecular phylogeny based on the small (SSU) and large subunit (LSU) ribosomal RNA genes clearly placed the new isolate within the “Nosema bombycis clade”. Considering both SSU and LSU concatenated partial sequences, the microsporidium from R. nu showed 99.5% nucleotide similarity with N. bombycis and 99.8% with its closest relative, a microsporidium isolated from Philosamia cynthia. According to its genetic and biological features, the R. nu isolate is proposed as the new species Nosema rachiplusiae sp. n., expanding the limited knowledge on microsporidia associated to endemic South-American moths.es_AR
dc.formatapplication/pdfeng
dc.language.isoeng
dc.publisherSpringer
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.sourceParasitology research 117 (5) : 1325–1331. (May 2018)eng
dc.subjectNosemaes_AR
dc.subjectRachiplusiaes_AR
dc.subjectUltraestructuraes_AR
dc.subjectHistopatologíaes_AR
dc.subjectFilogeniaes_AR
dc.subjectGenética Moleculares_AR
dc.subjectMolecular Geneticseng
dc.subjectPhylogenyeng
dc.subjectHistopathologyeng
dc.subjectUltrastructureeng
dc.subject.otherNosema Rachiplusiaees_AR
dc.titleBiological and molecular features of Nosema rachiplusiae sp. n., a microsporidium isolated from the neotropical moth Rachiplusia nu (Guenée) (Lepidoptera: Noctuidae)eng
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenInstituto de Microbiología y Zoología Agrícolaes_AR
dc.description.filFil: Arneodo Larochette, Joel Demian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Sciocco, Alicia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentinaes_AR
dc.subtypecientifico


Ficheros en el ítem

Thumbnail

Este ítem aparece en la(s) siguiente(s) colección(ones)

common

Mostrar el registro sencillo del ítem