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Abstract
Numerous species of legumes, including alfalfa, are critical factors for agroecosystems due to their ability to grow without nitrogen fertilizers derived from non-renewable fossil fuels, their contribution of organic nitrogen to the soil, and their increased nutritional value. Alfalfa is the main source of vegetable proteins in meat and milk production systems worldwide. Despite the economic and ecological importance of this autotetraploid and allogamous [ver mas...]
dc.contributor.authorJozefkowicz, Cintia
dc.contributor.authorFrare, Romina Alejandra
dc.contributor.authorOdorizzi, Ariel
dc.contributor.authorArolfo, Valeria
dc.contributor.authorPagano, Elba Maria
dc.contributor.authorBasigalup, Daniel Horacio
dc.contributor.authorAyub, Nicolás Daniel
dc.contributor.authorSoto, Gabriela Cinthia
dc.date.accessioned2018-06-12T12:46:57Z
dc.date.available2018-06-12T12:46:57Z
dc.date.issued2018-05
dc.identifier.issn0040-5752
dc.identifier.issn1432-2242
dc.identifier.otherhttps://doi.org/10.1007/s00122-018-3062-1
dc.identifier.urihttps://link.springer.com/article/10.1007%2Fs00122-018-3062-1
dc.identifier.urihttp://hdl.handle.net/20.500.12123/2599
dc.description.abstractNumerous species of legumes, including alfalfa, are critical factors for agroecosystems due to their ability to grow without nitrogen fertilizers derived from non-renewable fossil fuels, their contribution of organic nitrogen to the soil, and their increased nutritional value. Alfalfa is the main source of vegetable proteins in meat and milk production systems worldwide. Despite the economic and ecological importance of this autotetraploid and allogamous forage crop, little progress has been made in the incorporation of transgenic traits into commercial alfalfa. This is mainly due to the unusually strong transgene silencing and complex reproductive behavior of alfalfa, which limit the production of events with high transgene expression and the introgression of selected events within heterogeneous synthetic populations, respectively. In this report, we describe a novel procedure, called supertransgene process, where a glufosinate-tolerant alfalfa variety was developed using a single event containing the BAR transgene associated with an inversion. This approach can be used to maximize the expression of transgenic traits into elite alfalfa germplasm and to reduce the cost of production of transgenic alfalfa cultivars, contributing to the public improvement of this legume forage and other polyploid and outcrossing crop species.eng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.sourceTheoretical and applied genetics 131 (5) : 1111–1123. (May 2018)eng
dc.subjectMedicago Sativaes_AR
dc.subjectGermoplasmaes_AR
dc.subjectGermplasmeng
dc.subjectTransgénicoses_AR
dc.subjectTransgenicseng
dc.subjectVariedadeses_AR
dc.subjectVarietieseng
dc.subject.otherAlfalfaes_AR
dc.titleMaximizing the expression of transgenic traits into elite alfalfa germplasm using a supertransgene configuration in heterozygous conditionseng
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenInstituto de Genéticaes_AR
dc.description.filFil: Jozefkowicz, Cintia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina.es_AR
dc.description.filFil: Frare, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina.es_AR
dc.description.filFil: Odorizzi, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentinaes_AR
dc.description.filFil: Arolfo, Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentinaes_AR
dc.description.filFil: Pagano, Elba Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentinaes_AR
dc.description.filFil: Basigalup, Daniel Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentinaes_AR
dc.description.filFil: Ayub, Nicolás Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentinaes_AR
dc.description.filFil: Soto, Gabriela Cinthia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentinaes_AR
dc.subtypecientifico


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