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Abstract
In Argentina, glyphosate resistance was reported in a Lolium perenne population after 12 years of successful herbicide use. The aim of the current paper was to put in evidence for the mechanism of glyphosate resistance of this weed. Susceptible leaves treated with different doses of glyphosate and incubated in vitro showed an accumulation of shikimic acid of around three to five times the basal level, while no changes were detected in leaves of [ver mas...]
dc.contributor.authorYanniccari, Marcos Ezequiel
dc.contributor.authorGómez Lobato, María Eugenia
dc.contributor.authorIstilart, Carolina Maria
dc.contributor.authorNatalucci, Claudia
dc.contributor.authorGimenez, Daniel Oscar
dc.contributor.authorCastro, Ana Maria
dc.date.accessioned2018-05-08T13:14:03Z
dc.date.available2018-05-08T13:14:03Z
dc.date.issued2017-10
dc.identifier.issn2296-701X
dc.identifier.otherhttps://doi.org/10.3389/fevo.2017.00123
dc.identifier.urihttps://www.frontiersin.org/articles/10.3389/fevo.2017.00123/full
dc.identifier.urihttp://hdl.handle.net/20.500.12123/2347
dc.description.abstractIn Argentina, glyphosate resistance was reported in a Lolium perenne population after 12 years of successful herbicide use. The aim of the current paper was to put in evidence for the mechanism of glyphosate resistance of this weed. Susceptible leaves treated with different doses of glyphosate and incubated in vitro showed an accumulation of shikimic acid of around three to five times the basal level, while no changes were detected in leaves of glyphosate-resistant plants. The resistance mechanism prevents shikimate accumulation in leaves, even under such tissue-isolation conditions. The activity of the glyphosate target enzyme (EPSPS: 5-enolpyruvylshikimate-3-phosphate synthase) was quantified at different herbicide concentrations. EPSPS from resistant plants showed no difference in glyphosate-sensitivity compared to EPSPS from susceptible plants, and, accordingly, no amino acid substitution causing mutations associated with resistance were found. While the glyphosate target enzymes were equally sensitive, the basal EPSPS activity in glyphosate resistant plants was approximately 3-fold higher than the EPSPS activity in susceptible plants. This increased EPSPS activity in glyphosate resistant plants was associated with a 15-fold higher expression of EPSPS compared with susceptible plants. Therefore, the over-expression of EPSPS appears to be the main mechanism responsible for resistance to glyphosate. This mechanism has a constitutive character and has important effects on plant fitness, as recently reported.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourceFrontiers in Ecology and Evolution 5 : 123. (October 2017)es_AR
dc.subjectLolium Perennees_AR
dc.subjectHerbicidases_AR
dc.subjectHerbicideseng
dc.subjectResistencia a los Herbicidases_AR
dc.subjectResistance to Herbicideseng
dc.subjectGlifosatoes_AR
dc.subjectGlyphosateeng
dc.subject.otherRaigras perennees_AR
dc.subject.otherArgentinaes_AR
dc.titleMechanism of Resistance to Glyphosate in Lolium perenne from Argentinaes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEEA Barrowes_AR
dc.description.filFil: Yanniccari, Marcos Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Chacra Experimental Integrada Barrow; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Gómez Lobato, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentinaes_AR
dc.description.filFil: Istilart, Carolina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Chacra Experimental Integrada Barrow; Argentinaes_AR
dc.description.filFil: Natalucci, Claudia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación de Proteínas Vegetales (CIPROVE); Argentinaes_AR
dc.description.filFil: Gimenez, Daniel Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentinaes_AR
dc.description.filFil: Castro, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; Argentinaes_AR
dc.subtypecientifico


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