Mostrar el registro sencillo del ítem

resumen

Resumen
Neospora caninum infection is a major cause of abortion in cattle. The objectives of this study were to genetically characterize the N. caninum NC-6 Argentina isolate using a multilocus microsatellite analysis approach and to study its biological behavior by experimental inoculations into seronegative and seropositive pregnant cattle, evaluating the humoral and cellular immune response elicited and the occurrence of transplacental transmission and [ver mas...]
dc.contributor.authorBacigalupe, Diana
dc.contributor.authorBasso, W.
dc.contributor.authorCaspe, Sergio Gaston
dc.contributor.authorMore, Gastón
dc.contributor.authorLischinsky, Lilian Haydee
dc.contributor.authorGos, María Laura
dc.contributor.authorLeunda, Maria Rosa
dc.contributor.authorCampero, Lucía María
dc.contributor.authorMoore, Prando Dadin
dc.contributor.authorSchares, Gereon
dc.contributor.authorCampero, Carlos Manuel
dc.contributor.authorVenturini, María Cecilia
dc.date.accessioned2018-05-04T17:28:19Z
dc.date.available2018-05-04T17:28:19Z
dc.date.issued2013-07
dc.identifier.issn0932-0113
dc.identifier.issn1432-1955
dc.identifier.otherhttps://doi.org/10.1007/s00436-013-3424-1
dc.identifier.urihttps://link.springer.com/article/10.1007/s00436-013-3424-1
dc.identifier.urihttp://hdl.handle.net/20.500.12123/2329
dc.description.abstractNeospora caninum infection is a major cause of abortion in cattle. The objectives of this study were to genetically characterize the N. caninum NC-6 Argentina isolate using a multilocus microsatellite analysis approach and to study its biological behavior by experimental inoculations into seronegative and seropositive pregnant cattle, evaluating the humoral and cellular immune response elicited and the occurrence of transplacental transmission and fetopathy. Pregnant cows (65 days of gestation) seropositive and seronegative to N. caninum were intravenously inoculated with tachyzoites of the NC-6 Argentina N. caninum strain and slaughtered at 108 ± 2 days of gestation. Serum samples were analyzed for N. caninum antibodies by indirect fluorescent antibody test. The cellular immune response was analyzed by detection of gamma interferon (γIFN) production in blood cells. Tissue samples from dams, fetuses, and placental cotyledons were processed by histopathological and immunohistochemical techniques and examined for N. caninum DNA by PCR. Positive DNA samples were further analyzed by multilocus microsatellite typing for N. caninum. Inoculated animals had significantly higher N. caninum antibody titers and γIFN production than control animals. One seropositive inoculated cow aborted, one seronegative cow had a non-viable fetus, and the remaining fetuses from the experimentally inoculated dams had histopathologic lesions. The PCR was positive in 3/4 fetuses from seronegative inoculated cows and in 2/3 fetuses from seropositive inoculated cows. Multilocus microsatellite analysis revealed that the N. caninum DNA present in fetuses and placentas had an identical pattern to NC-6 Argentina strain. The NC-6 Argentina strain proved to be able to cross the placenta and to induce fetopathy in both the seropositive and seronegative dams.eng
dc.formatapplication/pdfeng
dc.language.isoenges_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.sourceParasitology research 112 (7) : 2585–2592. (July 2013)eng
dc.subjectNeospora caninumes_AR
dc.subjectGanado Bovinoes_AR
dc.subjectCattleeng
dc.subjectAbortoes_AR
dc.subjectAbortioneng
dc.subjectTécnicas Inmunológicases_AR
dc.subjectImmunological Techniqueseng
dc.subjectInoculaciónes_AR
dc.subjectInoculationeng
dc.subjectRespuesta Inmunológicaes_AR
dc.subjectImmune Responseeng
dc.subject.otherArgentina
dc.titleNeospora caninum NC-6 Argentina induces fetopathy in both serologically positive and negative experimentally inoculated pregnant damses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenEEA Mercedeses_AR
dc.description.origenEEA Balcarce
dc.description.filFil: Bacigalupe, Diana. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentinaes_AR
dc.description.filFil: Basso, W. University of Zurich. Vetsuisse Faculty. Institute of Parasitology; Suizaes_AR
dc.description.filFil: Caspe, Sergio Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentinaes_AR
dc.description.filFil: More, Gastón. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Lischinsky, Lilian Haydee. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentinaes_AR
dc.description.filFil: Gos, M.L. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentinaes_AR
dc.description.filFil: Leunda, Maria Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentinaes_AR
dc.description.filFil: Campero, Lucía María. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Moore, Prando Dadin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Schares, Gereon. Friedrich-Loeffler-Institut. Federal Research Institute for Animal Health. Institute of Epidemiology; Alemaniaes_AR
dc.description.filFil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentinaes_AR
dc.description.filFil: Venturini, María C. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentinaes_AR
dc.subtypecientifico


Ficheros en el ítem

Thumbnail

Este ítem aparece en la(s) siguiente(s) colección(ones)

common

Mostrar el registro sencillo del ítem