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Adh1 first intron polymorphisms in Argentinean populations of Ceratitis capitata

Abstract
DNA size polymorphisms were utilized in a study of 24 natural populations of Ceratitis capitata Wiedemann (Diptera: Tephritidae) from Argentina. The first intron of alcohol dehydrogenase 1 gene (Adh1) was amplified using exon priming intron crossing-polymerase chain reaction. Three size variants were detected among the 307 samples analyzed. To better differentiate the size variants, further digestion of PCR products with the EcoRI restriction enzyme [ver mas...]
DNA size polymorphisms were utilized in a study of 24 natural populations of Ceratitis capitata Wiedemann (Diptera: Tephritidae) from Argentina. The first intron of alcohol dehydrogenase 1 gene (Adh1) was amplified using exon priming intron crossing-polymerase chain reaction. Three size variants were detected among the 307 samples analyzed. To better differentiate the size variants, further digestion of PCR products with the EcoRI restriction enzyme was carried out. Complete nucleotide sequences of the three-allele variants were obtained and single changes, insertions, deletions, and EcoRI recognition sites were located. Population allele frequencies were analyzed and a global mean heterozygosity (He) of 0.33 was obtained. In most populations, observed allelic frequencies conformed to Hardy–Weinberg expectations. Significant differences between provinces and sampling sites within these provinces, and among some populations were found. The average number of insects exchanged among populations (Nm) was estimated and high values were observed between Argentina and populations fromtwo African countries (Morocco and Kenya), Australia, and Hawaii (Kauai). Pest introduction sources and dispersion patterns in Argentina are discussed based on these results as well as on available bibliographical data. [Cerrar]
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Author
Lanzavecchia, Silvia Beatriz;   Remis, Maria Isabel;   Cladera, Jorge Luis;   Zandomeni, Ruben;  
Fuente
Entomologia experimentalis et applicata 136 (1) : 53–65. (July 2010)
Date
2010-07
ISSN
0013-8703
1570-7458 (Online version)
URI
http://hdl.handle.net/20.500.12123/2227
DOI
https://doi.org/10.1111/j.1570-7458.2010.01002.x
Formato
pdf
Tipo de documento
artículo
Palabras Claves
Ceratitis Capitata; Alcohol Deshidrogenasa; Marcadores Genéticos; Intrones; Población; Tephritidae; Polimorfismo Genético; Genetic Polymorphism; Population; Introns; Genetic Markers; Alcohol Dehydrogenase; ADH1; Argentina;
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Excepto donde se diga explicitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
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