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Abstract
Plant viruses that are members of the Geminiviridae family have circular single-stranded DNA (ssDNA) genome and are responsible for major crop diseases worldwide. We have identified and characterized a novel monopartite geminivirus infecting tomato in Argentina. The full-length genome was cloned and sequenced. The genome-wide pairwise identity calculation that resulted in a maximum of 63% identity with all of other known geminiviruses indicated that it is [ver mas...]
dc.contributor.authorVaghi Medina, Carlos Gaston
dc.contributor.authorTeppa, Elin
dc.contributor.authorBornancini, Veronica Andrea
dc.contributor.authorFlores, Ceferino Rene
dc.contributor.authorMarino Buslje, Cristina
dc.contributor.authorLopez Lambertini, Paola Maria
dc.coverage.spatialArgentina (nation)
dc.date.accessioned2018-04-04T18:24:45Z
dc.date.available2018-04-04T18:24:45Z
dc.date.issued2018-01
dc.identifier.issn1664-302X
dc.identifier.otherhttps://doi.org/10.3389/fmicb.2017.02665
dc.identifier.urihttps://www.frontiersin.org/articles/10.3389/fmicb.2017.02665/full
dc.identifier.urihttp://hdl.handle.net/20.500.12123/2169
dc.description.abstractPlant viruses that are members of the Geminiviridae family have circular single-stranded DNA (ssDNA) genome and are responsible for major crop diseases worldwide. We have identified and characterized a novel monopartite geminivirus infecting tomato in Argentina. The full-length genome was cloned and sequenced. The genome-wide pairwise identity calculation that resulted in a maximum of 63% identity with all of other known geminiviruses indicated that it is a new geminivirus species. Biolistic infected plants presented interveinal yellowing, apical leaf curling and extreme root hypotrophy. Thus, the name proposed for this species is tomato apical leaf curl virus (ToALCV). The phylogenetic inferences suggested different evolutionary relationships for the replication-associated protein (Rep) and the coat protein (CP). Besides, the sequence similarity network (SSN) protein analyses showed that the complementary-sense gene products (RepA, Rep and C3) are similar to capulavirus while the viron-sense gene products (CP, MP and V3) are similar to topocuvirus, curtovirus and becurtovirus. Based on the data presented, ToALCV genome appears to have "modular organization" supported by its recombination origin. Analyses of the specificity-determining positions (SDPs) of the CP of geminiviruses defined nine subgroups that include geminiviruses that share the same type of insect vector. Our sequences were clustered with the sequences of topocuvirus, whose vector is the treehopper, Micrutalis malleifera. Also, a set of the highest scored amino acid residues was predicted for the CP, which could determine differences in virus transmission specificity. We predict that a treehopper could be the vector of ToALCV, but transmission assays need to be performed to confirm this. Given everything we demonstrate in this paper, ToALCV can be considered a type member of a new putative genus of the Geminiviridae family.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourceFrontiers in Microbiology January 2018es_AR
dc.subjectSolanum lycopersicumes_AR
dc.subjectCoat Proteinseng
dc.subjectRecombinationeng
dc.subjectTomatees_AR
dc.subject.otherTomatoeng
dc.subject.otherSequence Similarity Networkes_AR
dc.subject.otherTreehopperes_AR
dc.titleTomato apical leaf curl virus: a novel, monopartite geminivirus detected in tomatoes in Argentinaes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenINTA. CIAP. Instituto de Patología Vegetales_AR
dc.description.filFil: Vaghi Medina, Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentinaes_AR
dc.description.filFil:Teppa, Elin. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir; Argentinaes_AR
dc.description.filFil: Bornancini, Verónica A. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentinaes_AR
dc.description.filFil: Flores, Ceferino R. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Yuto; Argentinaes_AR
dc.description.filFil: Marino Buslje, Cristina. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir; Argentinaes_AR
dc.description.filFil: López Lambertini, Paola M. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentinaes_AR
dc.subtypecientifico


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