Show simple item record

resumen

Abstract
Background: Bovine leukemia virus (BLV) infection is omnipresent in dairy herds causing direct economic losses due to trade restrictions and lymphosarcoma-related deaths. Milk production drops and increase in the culling rate are also relevant and usually neglected. The BLV provirus persists throughout a lifetime and an inter-individual variation is observed in the level of infection (LI) in vivo. High LI is strongly correlated with disease progression [ver mas...]
dc.contributor.authorCarignano, Hugo Adrian
dc.contributor.authorRoldan, Dana Leticia
dc.contributor.authorBeribe, Maria Jose
dc.contributor.authorRaschia, Maria Agustina
dc.contributor.authorAmadio, Ariel
dc.contributor.authorNani, Juan Pablo
dc.contributor.authorGutierrez, Gerónimo
dc.contributor.authorAlvarez, Irene
dc.contributor.authorTrono, Karina Gabriela
dc.contributor.authorPoli, Mario Andres
dc.contributor.authorMiretti, Marcos Mateo
dc.date.accessioned2018-03-28T18:48:57Z
dc.date.available2018-03-28T18:48:57Z
dc.date.issued2018-02-13
dc.identifier.issn1471-2164
dc.identifier.otherhttps://doi.org/10.1186/s12864-018-4523-2
dc.identifier.urihttp://hdl.handle.net/20.500.12123/2150
dc.identifier.urihttps://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-018-4523-2
dc.description.abstractBackground: Bovine leukemia virus (BLV) infection is omnipresent in dairy herds causing direct economic losses due to trade restrictions and lymphosarcoma-related deaths. Milk production drops and increase in the culling rate are also relevant and usually neglected. The BLV provirus persists throughout a lifetime and an inter-individual variation is observed in the level of infection (LI) in vivo. High LI is strongly correlated with disease progression and BLV transmission among herd mates. In a context of high prevalence, classical control strategies are economically prohibitive. Alternatively, host genomics studies aiming to dissect loci associated with LI are potentially useful tools for genetic selection programs tending to abrogate the viral spreading. The LI was measured through the proviral load (PVL) set–point and white blood cells (WBC) counts. The goals of this work were to gain insight into the contribution of SNPs (bovine 50KSNP panel) on LI variability and to identify genomics regions underlying this trait. Results: We quantified anti–p24 response and total leukocytes count in peripheral blood from 1800 cows and used these to select 800 individuals with extreme phenotypes in WBCs and PVL. Two case-control genomic association studies using linear mixed models (LMMs) considering population stratification were performed. The proportion of the variance captured by all QC-passed SNPs represented 0.63 (SE ± 0.14) of the phenotypic variance for PVL and 0. 56 (SE ± 0.15) for WBCs. Overall, significant associations (Bonferroni’s corrected -log10p > 5.94) were shared for both phenotypes by 24 SNPs within the Bovine MHC. Founder haplotypes were used to measure the linkage disequilibrium (LD) extent (r2 = 0.22 ± 0.27 at inter-SNP distance of 25−50 kb). The SNPs and LD blocks indicated genes potentially associated with LI in infected cows: i.e. relevant immune response related genes (DQA1, DRB3, BOLA-A, LTA, LTB, TNF, IER3, GRP111, CRISP1), several genes involved in cell cytoskeletal reorganization (CD2AP, PKHD1, FLOT1, TUBB5) and modelling of the extracellular matrix (TRAM2, TNXB). Host transcription factors (TFs) were also highlighted (TFAP2D; ABT1, GCM1, PRRC2A). Conclusions: Data obtained represent a step forward to understand the biology of BLV–bovine interaction, and provide genetic information potentially applicable to selective breeding programseng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.rightsinfo:eu-repo/semantics/openAccesseng
dc.sourceBMC Genomics 19 :142. (2018)eng
dc.subjectGanado de Lechees_AR
dc.subjectLeucemiaes_AR
dc.subjectBovinaes_AR
dc.subjectGenomases_AR
dc.subjectVirus Leucemia Bovinaes_AR
dc.subjectGenetic Polymorphismeng
dc.subjectBovine Leukaemia Viruseng
dc.subjectLeukaemiaeng
dc.subjectGenomeseng
dc.subjectDairy Cattleeng
dc.subject.otherSNPes_AR
dc.subject.otherPolimorfismos de Nucleótido Unicoes_AR
dc.titleGenome-wide scan for commons SNPs affecting bovine leukemia virus infection level in dairy cattleeng
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.filFil: Carignano, Hugo Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentinaes_AR
dc.description.filFil: Roldan, Dana Leticia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentinaes_AR
dc.description.filFil: Beribe, Maria Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino; Argentinaes_AR
dc.description.filFil: Raschia, Maria Agustina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentinaes_AR
dc.description.filFil: Amadio, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Nani, Juan Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentinaes_AR
dc.description.filFil: Gutierrez, Gerónimo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentinaes_AR
dc.description.filFil: Alvarez, Irene. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Trono, Karina Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentinaes_AR
dc.description.filFil: Poli, Mario Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentinaes_AR
dc.description.filFil: Miretti, Marcos Mateo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina. Universidad Nacional de Misiones. Instituto de Biología Subtropical - Nodo Posadas; Argentinaes_AR
dc.subtypecientifico


Files in this item

Thumbnail

This item appears in the following Collection(s)

common

Show simple item record