Show simple item record

resumen

Abstract
Wheat vernalization requirement is mainly controlled by the VRN1, VRN2, VRN3, and VRN4 genes. The first three have been cloned and have homoeologs in all three genomes. VRN4 has been found only in the D genome (VRN-D4) and has not been cloned. We constructed a high-density genetic map of the VRN-D4 region and mapped VRN-D4 within a 0.09 cM interval in the centromeric region of chromosome 5D. Using telocentric 5D chromosomes generated from the VRN-D4 donor [ver mas...]
dc.contributor.authorKippes, Néstor Fabián
dc.contributor.authorZhu, Jie
dc.contributor.authorChen, Andrew
dc.contributor.authorVanzetti, Leonardo Sebastian
dc.contributor.authorLukaszewski, Adam
dc.contributor.authorNishida, Hidetaka
dc.contributor.authorKato, Kenji
dc.contributor.authorDvorak, Jan
dc.contributor.authorDubcovsky, Jorge
dc.date.accessioned2018-03-23T17:35:13Z
dc.date.available2018-03-23T17:35:13Z
dc.date.issued2014-02
dc.identifier.issn1617-4615 (Print)
dc.identifier.issn1617-4623 (Online)
dc.identifier.otherhttps://doi.org/10.1007/s00438-013-0788-y
dc.identifier.urihttps://link.springer.com/article/10.1007/s00438-013-0788-y
dc.identifier.urihttp://hdl.handle.net/20.500.12123/2111
dc.identifier.urihttp://ri.conicet.gov.ar/handle/11336/28515
dc.description.abstractWheat vernalization requirement is mainly controlled by the VRN1, VRN2, VRN3, and VRN4 genes. The first three have been cloned and have homoeologs in all three genomes. VRN4 has been found only in the D genome (VRN-D4) and has not been cloned. We constructed a high-density genetic map of the VRN-D4 region and mapped VRN-D4 within a 0.09 cM interval in the centromeric region of chromosome 5D. Using telocentric 5D chromosomes generated from the VRN-D4 donor Triple Dirk F, we determined that VRN-D4 is located on the short arm. The VRN-D4 candidate region is colinear with a 2.24 Mb region on Brachypodium distachyon chromosome 4, which includes 127 predicted genes. Ten of these genes have predicted roles in development but we detected no functional polymorphisms associated to VRN-D4. Two recombination events separated VRN-D4 from TaVIL-D1, the wheat homolog of Arabidopsis vernalization gene VIL1, confirming that this gene is not a candidate for VRN-D4. We detected significant interactions between VRN-D4 and other four genes controlling vernalization requirement (Vrn-A1, Vrn-B1, Vrn-D1, and Vrn-B3), which confirmed that VRN-D4 is part of the vernalization pathway and that it is either upstream or is part of the regulatory feedback loop involving VRN1, VRN2 and VRN3 genes. The precise mapping of VRN-D4 and the characterization of its interactions with other vernalization genes provide valuable information for the utilization of VRN-D4 in wheat improvement and for our current efforts to clone this vernalization gene.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourceMolecular Genetics and Genomics 289 (1) : 47–62 (February 2014)es_AR
dc.subjectTrigoes_AR
dc.subjectWheateng
dc.subjectVernalizaciónes_AR
dc.subjectVernalizationeng
dc.subjectMapas Genéticoses_AR
dc.subjectGenetic Mapseng
dc.subjectHexaploidiaes_AR
dc.subjectHexaploidyeng
dc.titleFine mapping and epistatic interactions of the vernalization gene VRN-D4 in hexaploid wheates_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEEA Marcos Juárezes_AR
dc.description.filFil: Kippes, Néstor Fabián. University of California at Davis. Department of Plant Sciences; Estados Unidoses_AR
dc.description.filFil: Zhu, Jie. Washington State University. USDA-ARS Wheat Genetics, Quality, Physiology and Disease Research Unit; Estados Unidoses_AR
dc.description.filFil: Chen, Andrew. University of California at Davis. Department of Plant Sciences; Estados Unidoses_AR
dc.description.filFil: Vanzetti, Leonardo Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez. Grupo Biotecnología y Recursos Genéticos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Lukaszewski, Adam. University of California. Department of Botany and Plant Sciences; Estados Unidoses_AR
dc.description.filFil: Nishida, Hidetaka. Okayama University. Graduate School of Environmental and Life Science; Japónes_AR
dc.description.filFil: Kato, Kenji. Okayama University. Graduate School of Environmental and Life Science; Japónes_AR
dc.description.filFil: Dvorak, Jan. University of California at Davis. Department of Plant Sciences; Estados Unidoses_AR
dc.description.filFil: Dubcovsky, Jorge. University of California at Davies. Department of Plant Sciences; Estados Unidos. Howard Hughes Medical Institute; Estados Unidoses_AR
dc.subtypecientifico


Files in this item

Thumbnail

This item appears in the following Collection(s)

common

Show simple item record